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Literature summary for 6.3.2.4 extracted from
- Isolation, cloning, and sequencing of the Salmonella typhimurium ddlA gene with purification and characterization of its product, D-alanine:D-alanine ligase (ADP forming) (1988), Biochemistry, 27, 3701-3708.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned behind the tac promoter in the plasmid pKK223-3, expression in Escherichia coli JM105 | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39271 | - |
x * 39271, calculation from nucleotide sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
| 42000 | - |
x * 42000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overproducing strain | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.4 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-Ala + D-Ala | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + D-Ala-D-Ala | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| ? | x * 39271, calculation from nucleotide sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 63 | - |
5 min, complete inactivation | Salmonella enterica subsp. enterica serovar Typhimurium |
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