Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cadmium aerosols | 2.4 mg Cd/m3, enhance in the lung the expression of the enzyme's heavy, catalytic subunit gamma-GCS-HS by 4.5fold after 15 min, 8fold after 6 h, increase in enzyme activity and GSH production rate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-buthionine-SR-sulfoximine | specific | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
x * 73000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | Rattus norvegicus | first and rate limiting step in GSH de novo biosynthesis | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Lewis rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alveolus | corners of, highest enzyme content | Rattus norvegicus | - |
epithelium | alveolar, higher content compared to the lung parenchyma | Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
additional information | pulmonary distribution | Rattus norvegicus | - |
parenchyma | lower content compared to the alveolar epithelium | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0027 | - |
lung extract exposed to air | Rattus norvegicus |
0.0041 | - |
lung extract exposed to cadmium aerosol with 3.3 mg Cd/m3 | Rattus norvegicus |
0.0048 | - |
lung extract exposed to cadmium aerosol with 4.2 mg Cd/m3 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | first and rate limiting step in GSH de novo biosynthesis | Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
gamma-GCS | - |
Rattus norvegicus |
gamma-Glutamylcysteine synthetase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |