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Literature summary for 6.3.2.13 extracted from

  • Munshi, T.; Gupta, A.; Evangelopoulos, D.; Guzman, J.D.; Gibbons, S.; Keep, N.H.; Bhakta, S.
    Characterisation of ATP-dependent Mur ligases involved in the biogenesis of cell wall peptidoglycan in Mycobacterium tuberculosis (2013), PLoS ONE, 8, e60143.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.13

Cloned(Commentary)

Cloned (Comment) Organism
gene murE or Rv2158c, located in the division/cell wall (dcw) cluster,, identification of a promoter driving the co-transcription of mur synthetases along with key cell division genes such as ftsQ and ftsW, recombinant expression of His6-tagged enzyme in Escherichia coli BL21(DE3)/pLysS and Pseudomonas putida KT2442, subcloning in Escherichia coli strain DH5alpha.. Coexpression of genes murC/D/E/F/nat and genes pknA, pknB, murI, dapF, ddlA, namH, Rv2160c, ftsW, ftsQ, ftsZ, sepF, wag31 in Mycobacterium smegmatis Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Mycobacterium tuberculosis 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially substitute for Mg2+ Mycobacterium tuberculosis
Mg2+ required Mycobacterium tuberculosis
Mn2+ can substitute for Mg2+ Mycobacterium tuberculosis
Zn2+ can partially substitute for Mg2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate Mycobacterium tuberculosis
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate Mycobacterium tuberculosis H37Rv
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
additional information Mycobacterium tuberculosis MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP ?
-
 ?
additional information Mycobacterium tuberculosis H37Rv MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WJL3 gene murE or Rv2158c
-
Mycobacterium tuberculosis H37Rv P9WJL3 gene murE or Rv2158c
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
-
 Mycobacterium tuberculosis ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
additional information MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP Mycobacterium tuberculosis ?
-
 ?
additional information MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
Mur ligase
-
 Mycobacterium tuberculosis
MurE
-
 Mycobacterium tuberculosis
MurE synthetase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 purified recombinant His-tagged enzyme, stable up to Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
additional information protein-protein interaction network of MurC, D, E, and F synthetases, overview Mycobacterium tuberculosis
physiological function ATP-dependent Mur ligases play essential roles in the biosynthesis of cell wall peptidoglycan as they catalyze the ligation of key amino acid residues to the stem peptide at the expense of ATP hydrolysis Mycobacterium tuberculosis