Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | activation of mutant K202A | Escherichia coli | |
Butyrate | activation of mutant K202A | Escherichia coli | |
ethylamine | slight activation of mutant K202A | Escherichia coli | |
formate | slight activation of mutant K202A | Escherichia coli | |
propionate | strong activation of mutant K202A | Escherichia coli | |
Propylamine | slight activation of mutant K202A | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and MurF mutant K202A as His-tagged enzymes in strain JM83 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K202A | site-directed mutagenesis, exchange of highly conserved lysine residue leads to highly reduced activity, activity can be rescued best by addition of propionate or other short-chain carboxylic acids, but only in a small extent by amines | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | inhibition of wild-type enzyme | Escherichia coli | |
Butylamine | inhibition of wild-type and mutant K202A; inhibition of wild-type enzyme | Escherichia coli | |
Butyrate | slight inhibition of wild-type enzyme | Escherichia coli | |
ethylamine | inhibition of wild-type enzyme | Escherichia coli | |
formate | inhibition of wild-type enzyme | Escherichia coli | |
methylamine | inhibition of wild-type enzyme | Escherichia coli | |
propionate | inhibition of wild-type enzyme | Escherichia coli | |
Propylamine | inhibition of wild-type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | Escherichia coli | - |
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzyme from strain JM83 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.006 | - |
purified recombinant mutant K202A, in presence of butylamine | Escherichia coli |
0.016 | - |
purified recombinant mutant K202A | Escherichia coli |
0.032 | - |
purified recombinant mutant K202A, in presence of propylamine | Escherichia coli |
0.046 | - |
purified recombinant mutant K202A, in presence of ethylamine | Escherichia coli |
0.052 | - |
purified recombinant mutant K202A, in presence of formate | Escherichia coli |
0.437 | - |
purified recombinant mutant K202A, in presence of butyrate | Escherichia coli |
0.511 | - |
purified recombinant mutant K202A, in presence of acetate | Escherichia coli |
0.55 | - |
purified recombinant wild-type enzyme, in presence of methylamine | Escherichia coli |
0.71 | - |
purified recombinant wild-type enzyme, in presence of propylamine | Escherichia coli |
0.72 | - |
purified recombinant wild-type enzyme, in presence of butylamine | Escherichia coli |
0.76 | - |
purified recombinant wild-type enzyme, in presence of propionate | Escherichia coli |
0.769 | - |
purified recombinant mutant K202A, in presence of propionate | Escherichia coli |
0.8 | - |
purified recombinant wild-type enzyme, in presence of ethylamine | Escherichia coli |
0.88 | - |
purified recombinant wild-type enzyme, in presence of acetate | Escherichia coli |
0.93 | - |
purified recombinant wild-type enzyme, in presence of formate | Escherichia coli |
1.24 | - |
purified recombinant wild-type enzyme, in presence of butyrate | Escherichia coli |
1.27 | - |
purified recombinant wild-type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-Ala-D-Ala-adding enzyme | - |
Escherichia coli |
Synthetase, uridine diphosphoacetylmuramoylpentapeptide | - |
Escherichia coli |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase | - |
Escherichia coli |
UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase | - |
Escherichia coli |
UDPacetylmuramoylpentapeptide synthetase | - |
Escherichia coli |
Uridine diphosphoacetylmuramoylpentapeptide synthetase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |