Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
structures of the apoenzyme and the reaction intermediate complex are determined by X-ray crystallography to resolutions of 2.5 A and 1.85 A, respectively. Structural analysis indicate that the apoenzyme adopts an open and relatively mobile structure, while the complex structure is closed and entirely rigid. In the complex structure, pantothenate synthetase and acetate are bound in the active site. Acetate might mimic the substrate beta-alanine. Therefore, the complex structure might represent a catalytic state poised for in-line nucleophilic attack on pantothenate synthetase | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q2FV22 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Staphylococcus aureus | AMP + diphosphate + (R)-pantothenate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pantothenate synthetase | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
beta-Alanine | co-substrate: (R)-pantoate, pH 8.0, 30°C | Staphylococcus aureus | |
1.5 | - |
(R)-pantoate | co-substrate: beta-alanine, pH 8.0, 30°C | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Staphylococcus aureus |