Literature summary for 6.3.1.5 extracted from

Muratsubaki, H.; Enomoto, K.; Soejima, A.; Satake, K.
An enzyme cycling method for measurement of allantoin in human serum (2008), Anal. Biochem., 378, 65-70.

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Application

Application	Comment	Organism
analysis	method for measurement of allantoin in human serum. Serum allantoin is converted to allantoate by the action of allantoinase,and endogenous ammonia is simultaneously removed by the action of glutamine synthetase II. In the second step, L-methionine sulfoximine is used to inhibit glutamine synthetase II, and ammonia is liberated from allantoate by the activity of allantoate amidohydrolase. The ammonia is then converted to NAD by NAD synthetase. Subsequent action of glucose dehydrogenase and diaphorase acts to cycle the formed NAD between its oxidized and reduced forms, resulting in the production of WST-1 formazan, which is monitored at 450 nm. The assay standard curve is linear from 0 to 70 microM allantoin	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	commercial preparation	-

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Release 2023.1 (January 2023)