Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
deamido-NAD+ | in 60 mM Tris-HCl pH 8.0, 10 mM NH4Cl, 10 mM MgCl2, 10 mM KCl | Staphylococcus aureus | |
0.2 | - |
ATP | in 60 mM Tris-HCl pH 8.0, 10 mM NH4Cl, 10 mM MgCl2, 10 mM KCl | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
strain 8325-4 | - |
Staphylococcus aureus NCTC 8325 | - |
strain 8325-4 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA Superflow resin chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | - |
Staphylococcus aureus | AMP + diphosphate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | - |
Staphylococcus aureus NCTC 8325 | AMP + diphosphate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD synthetase | - |
Staphylococcus aureus |
NadE | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |