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Literature summary for 6.3.1.5 extracted from

  • Stockman, B.J.; Lodovice, I.J.; Fisher, D.A.; McColl, A.S.; Xie, Z.
    A nuclear magnetic resonance-based functional assay for nicotinamide adenine dinucleotide synthetase (2007), J. Biomol. Screen., 12, 457-463.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
deamido-NAD+ in 60 mM Tris-HCl pH 8.0, 10 mM NH4Cl, 10 mM MgCl2, 10 mM KCl Staphylococcus aureus
0.2
-
ATP in 60 mM Tris-HCl pH 8.0, 10 mM NH4Cl, 10 mM MgCl2, 10 mM KCl Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
strain 8325-4
-
Staphylococcus aureus NCTC 8325
-
strain 8325-4
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Superflow resin chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + NH3
-
Staphylococcus aureus AMP + diphosphate + NAD+
-
?
ATP + deamido-NAD+ + NH3
-
Staphylococcus aureus NCTC 8325 AMP + diphosphate + NAD+
-
?

Synonyms

Synonyms Comment Organism
NAD synthetase
-
Staphylococcus aureus
NadE
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Staphylococcus aureus