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Literature summary for 6.3.1.2 extracted from

  • Adul Rahman, R.N.; Jongsareejit, B.; Fujiwara, S.; Imanaka, T.
    Characterization of recombinant glutamine synthetase from the hyperthermophilic archaeon Pyrococcus sp. strain KOD1 (1997), Appl. Environ. Microbiol., 63, 2472-2476.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ME8459 (glnA mutant strain) Pyrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
hydroxylamine the Km value of GlnA for hydroxylamine is higher when a high concentration was used (5 to 30mM), 60°C, pH 7.8 Pyrococcus sp.
23.5
-
L-glutamate 60°C, pH 7.8 Pyrococcus sp.
28
-
ATP 60°C, pH 7.8 Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
637000
-
gel filtration Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp. O08467
-
-
Pyrococcus sp. KOD1 O08467
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + hydroxylamine
-
Pyrococcus sp. ?
-
?
ATP + L-glutamate + hydroxylamine
-
Pyrococcus sp. KOD1 ?
-
?
additional information in reactions in which Mn2+ is used, GTP, UTP, and CTP havea limited ability to replace ATP (less than 30%), while in reactions in which Mg2+ was used, complete inhibition by these NTPs is observed. Broad NTP specificity of hyperthermophilic archaeon synthetases suggests that enzymes of ancestral life forms can utilize various NTPs besides ATP Pyrococcus sp. ?
-
?
additional information in reactions in which Mn2+ is used, GTP, UTP, and CTP havea limited ability to replace ATP (less than 30%), while in reactions in which Mg2+ was used, complete inhibition by these NTPs is observed. Broad NTP specificity of hyperthermophilic archaeon synthetases suggests that enzymes of ancestral life forms can utilize various NTPs besides ATP Pyrococcus sp. KOD1 ?
-
?

Subunits

Subunits Comment Organism
dodecamer
-
Pyrococcus sp.

Synonyms

Synonyms Comment Organism
GlnA
-
Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pyrococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 60% of maximal activity, 70°C: about 40% of maximal activity Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life: 98 min Pyrococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.83
-
hydroxylamine 60°C, pH 7.8 Pyrococcus sp.
36.5
-
L-glutamate 60°C, pH 7.8 Pyrococcus sp.
52.7
-
ATP 60°C, pH 7.8 Pyrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Pyrococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8.2 pH 7.0: about 60% of maximal activity, pH 8.2: about 70% of maximal activity Pyrococcus sp.

General Information

General Information Comment Organism
physiological function hyperthermophilic archaea do not utilize glutamine synthetase predominantly for ammonia assimilation (the major pathway for ammonia assimilation is through glutamate dehydrogenase). The enzyme might play some role in ammonia assimilation under ammonia-starvation conditions Pyrococcus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
hydroxylamine the Km value of GlnA for hydroxylamine is higher when a high concentration was used (5 to 30mM), 60°C, pH 7.8 Pyrococcus sp.
1.6
-
L-glutamate 60°C, pH 7.8 Pyrococcus sp.
1.9
-
ATP 60°C, pH 7.8 Pyrococcus sp.