Crystallization (Comment) | Organism |
---|---|
complex of the enzyme with the purine analogue 1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-dione alone to 2.55 A resolution, and in complex with 1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-dione together with methionine sulfoximine phosphate, magnesium and phosphate, to 2.2 A resolution. The former represents a relaxed, inactive conformation of the enzyme, while the latter is a taut, active one. Compound 1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-dione binds at the same position in the nucleotide site, regardless of the conformational state | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-dione | - |
Mycobacterium tuberculosis | |
DMSO | about 15% activation at 2% | Mycobacterium tuberculosis | |
Mg2+ | activiation up to 25 mM, inhibition above | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
ATP | pH 7.5 | Mycobacterium tuberculosis | |
10.8 | - |
NH4+ | pH 7.5 | Mycobacterium tuberculosis | |
21.6 | - |
glutamate | pH 7.5 | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation up to 25 mM, inhibition above | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WN39 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WN39 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | - |
Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + glutamate + NH4+ | - |
Mycobacterium tuberculosis H37Rv | ADP + phosphate + L-glutamine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0025 | - |
- |
Mycobacterium tuberculosis | 1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-dione |