Any feedback?
Literature summary for 6.3.1.2 extracted from
- Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes (2008), Proc. Natl. Acad. Sci. USA, 105, 1014-1019.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E424K | the mutant enzyme is defective in its ability to interact with GlnR | Bacillus subtilis |
| S186F | the mutant enzyme is defective in its ability to interact with GlnR | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-glutamine | the enzyme is feedback inhibited, the feedback inhibition of GS induces the sequence-specific binding of transcription factor GlnR to DNA in nitrogen metabolism regulation by 32fold and reduces the dissociation rate by 18fold stabilizing the complexes, overview | Bacillus subtilis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, about, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + NH3 | Bacillus subtilis | - |
ADP + phosphate + L-glutamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + NH3 | - |
Bacillus subtilis | ADP + phosphate + L-glutamine | - |
? | |
| additional information | the feedback-inhibited enzyme, acting as a chaperone, is required for the binding of transcription factor GlnR to DNA, the feedback inhibition of GS by L-glutamine induces the sequence-specific binding of transcription factor GlnR to DNA in nitrogen metabolism regulation by 32fold and reduces the dissociation rate by 18fold stabilizing the complexes, overview | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, about, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutamine synthetase | - |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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