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Literature summary for 6.3.1.2 extracted from
- Molecular cloning and characterization of a cytosolic glutamine synthetase gene, a fiber strength-associated gene in cotton (2008), Planta, 228, 473-483.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GhGS, DNA and amino acid sequence determination and analysis, molecular mapping of the GhGS gene, tissue-specific expression analysis | Gossypium hirsutum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | identification of single nucleotide polymorphisms of the genomic GhGS sequences in 7235 and TM-1 | Gossypium hirsutum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | isozyme GS2 | Gossypium hirsutum | 9507 | - |
| cytosol | isozyme GS1, the enzyme is fiber associated | Gossypium hirsutum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Gossypium hirsutum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + NH3 | Gossypium hirsutum | - |
ADP + phosphate + L-glutamine | - |
? | |
| additional information | Gossypium hirsutum | GS is involved in the assimilation of ammonia in the plant. GS catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gossypium hirsutum | A8WCU3 | gene GhGS; allotetraploid, strains 7235 and TM-1, gene GhGS, isozymes GS1 and GS2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fiber | association with | Gossypium hirsutum | - |
| leaf | - |
Gossypium hirsutum | - |
| ovum | - |
Gossypium hirsutum | - |
| root | - |
Gossypium hirsutum | - |
| stem | - |
Gossypium hirsutum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity and expression of GS during ovule and fiber development, overview | Gossypium hirsutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + NH3 | - |
Gossypium hirsutum | ADP + phosphate + L-glutamine | - |
? | |
| additional information | GS is involved in the assimilation of ammonia in the plant. GS catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine | Gossypium hirsutum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | - |
Gossypium hirsutum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutamine synthetase | - |
Gossypium hirsutum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gossypium hirsutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Gossypium hirsutum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Gossypium hirsutum | |
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