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Literature summary for 6.3.1.2 extracted from

  • Rueda-Lopez, M.; Crespillo, R.; Canovas, F.M.; Avila, C.
    Differential regulation of two glutamine synthetase genes by a single Dof transcription factor (2008), Plant J., 56, 73-85.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information transcription factor PpDof5 activates transcription of isozymes GS1a and GS1b, it is involved in enzyme expression regulation in the whole plant and during thr whole development and ia expressed itself with low levels of gene expression in green tissues and much higher levels in roots and lignified shoots, high levels in germinating seedlings, overview Pinus pinaster

Cloned(Commentary)

Cloned (Comment) Organism
isozymes GS1a and GS1b, expression analysis in leaves Nicotiana benthamiana

Protein Variants

Protein Variants Comment Organism
additional information recombinant transient expression of transcription factor PpDof5 from Pinus pinaster, using infiltration with Agrobacterium tumefaciens cells, the recombinant protein regulates the expression of isozymes GS1a and GS1b promoters in tobacco leaves, overview Nicotiana benthamiana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Nicotiana benthamiana 5829
-
cytosol
-
Pinus pinaster 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Nicotiana benthamiana
Mg2+
-
Pinus pinaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + NH3 Nicotiana benthamiana
-
ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 Pinus pinaster the enzyme is involved in regulation of ammonium assimilation requirements during tree development, regulatory mechanism for the transcriptional control of the spatial distribution of cytosolic GS isoforms in pine, overview ADP + phosphate + L-glutamine
-
?
additional information Pinus pinaster PpDof5 has an antagonistic regulatory function in the expression of GS1a and GS1b promoters in pine protoplasts transfected with the transcription factor under control of CaMV 35S promoter, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana
-
isozymes GS1a and GS1b
-
Pinus pinaster P81107 isozymes GS1a and GS1b
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana benthamiana
-
leaf
-
Pinus pinaster
-
additional information expression patterns and regulatory model of GS1 gene expression in pine Pinus pinaster
-
root
-
Pinus pinaster
-
seedling germinating Pinus pinaster
-
shoot
-
Pinus pinaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH3
-
Nicotiana benthamiana ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3
-
Pinus pinaster ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 the enzyme is involved in regulation of ammonium assimilation requirements during tree development, regulatory mechanism for the transcriptional control of the spatial distribution of cytosolic GS isoforms in pine, overview Pinus pinaster ADP + phosphate + L-glutamine
-
?
additional information PpDof5 has an antagonistic regulatory function in the expression of GS1a and GS1b promoters in pine protoplasts transfected with the transcription factor under control of CaMV 35S promoter, overview Pinus pinaster ?
-
?

Synonyms

Synonyms Comment Organism
Glutamine synthetase
-
Nicotiana benthamiana
Glutamine synthetase
-
Pinus pinaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nicotiana benthamiana
ATP
-
Pinus pinaster