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Literature summary for 6.3.1.2 extracted from
- Post-translational regulation of cytosolic glutamine synthetase of Medicago truncatula (2006), J. Exp. Bot., 57, 2751-2761.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Medicago truncatula |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
L-Glu | phosphorylated GS1a, in the presence of 0.0005 mM microcystin | Medicago truncatula |  |
| 2.8 | - |
L-Glu | non-phosphorylated GS1a, in the presence of 0.0005 mM microcystin | Medicago truncatula | |
| 2.9 | - |
ATP | phosphorylated GS1a, in the presence of 0.0005 mM microcystin | Medicago truncatula | |
| 3 | - |
ATP | non-phosphorylated GS1a, in the presence of 0.0005 mM microcystin | Medicago truncatula | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Medicago truncatula | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE, GS2 | Medicago truncatula |
| 41500 | - |
SDS-PAGE, His-tagged GS1a | Medicago truncatula |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago truncatula | - |
cultivar Jemalong J5 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation in root nodules | Medicago truncatula |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity chromatography and P10 Sephadex column chromatography | Medicago truncatula |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Medicago truncatula | - |
| nodule | - |
Medicago truncatula | - |
| root | - |
Medicago truncatula | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + NH4+ | - |
Medicago truncatula | ADP + phosphate + L-Gln | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutamine synthetase | - |
Medicago truncatula |
| GS1 kinase | - |
Medicago truncatula |
| GS1a | - |
Medicago truncatula |
| GS2 | - |
Medicago truncatula |
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Release 2023.1 (January 2023)
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