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Literature summary for 6.3.1.2 extracted from

  • Ishiyama, K.; Inoue, E.; Yamaya, T.; Takahashi, H.
    Gln-49 and Ser-174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase (2005), Plant Cell Physiol., 47, 299-303.
    View publication on PubMed
Search for more literature for 6.3.1.2

Protein Variants

Protein Variants Comment Organism
A174S isoenzyme GLN1,3, 3.6fold increase in ratio of turnover-number to KM-value for NH4+ Arabidopsis thaliana
K49Q isoenzyme GLN1,3, 3fold increase in ratio of turnover-number to KM-value for NH4+ Arabidopsis thaliana
K49Q/A174S isoenzyme GLN1,3, 2.8fold increase in ratio of turnover-number to KM-value for NH4+ Arabidopsis thaliana
Q49K isoenzyme GLN1,4, 2.2fold decrease in ratio of turnover-number to KM-value for NH4+ Arabidopsis thaliana
Q49K/S174A isoenzyme GLN1,4, 6.8fold increase in ratio of turnover-number to KM-value for NH4+ Arabidopsis thaliana
S174A isoenzyme GLN1,4, 1.3fold increase in ratio of turnover-number to KM-value for NH4+ Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.119
-
 NH4+ mutant A174S of isoenzyme GLN1,4 Arabidopsis thaliana
0.12
-
 NH4+ isoenzyme GLN1,4 Arabidopsis thaliana
0.334
-
 NH4+ mutant A174S of isoenzyme GLN1,3 Arabidopsis thaliana
0.45
-
 NH4+ mutant K49Q of isoenzyme GLN1,3 Arabidopsis thaliana
0.453
-
 NH4+ mutant K49Q of isoenzyme GLN1,4 Arabidopsis thaliana
0.456
-
 NH4+ mutant K49Q/A174S of isoenzyme GLN1,3 Arabidopsis thaliana
0.67
-
 L-Glu isoenzyme GLN1,4 Arabidopsis thaliana
0.736
-
 NH4+ mutant K49Q/A174S of isoenzyme GLN1,4 Arabidopsis thaliana
0.83
-
 L-Glu mutant A174S of isoenzyme GLN1,4 Arabidopsis thaliana
1.14
-
 L-Glu mutant K49Q of isoenzyme GLN1,4 Arabidopsis thaliana
1.33
-
 NH4+ isoenzyme GLN1,3 Arabidopsis thaliana
1.43
-
 L-Glu mutant K49Q/A174S of isoenzyme GLN1,4 Arabidopsis thaliana
2.4
-
 L-Glu mutant K49Q/A174S of isoenzyme GLN1,3 Arabidopsis thaliana
3.2
-
 L-Glu mutant A174S of isoenzyme GLN1,3 Arabidopsis thaliana
3.2
-
 L-Glu mutant K49Q of isoenzyme GLN1,3 Arabidopsis thaliana
4.17
-
 L-Glu isoenzyme GLN1,3 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 isoenzyme GLN1,4
-
Arabidopsis thaliana Q9LVI8 isoenzyme GLN1,3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Arabidopsis thaliana ADP + phosphate + L-Gln
-
 ?

Synonyms

Synonyms Comment Organism
GLN1,3
-
 Arabidopsis thaliana
GLN1,4
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.16
-
 NH4+ mutant A174S of isoenzyme GLN1,4 Arabidopsis thaliana
2.29
-
 L-Glu mutant A174S of isoenzyme GLN1,4 Arabidopsis thaliana
2.81
-
 NH4+ mutant K49Q/A174S of isoenzyme GLN1,4 Arabidopsis thaliana
2.96
-
 NH4+ isoenzyme GLN1,4 Arabidopsis thaliana
2.97
-
 L-Glu mutant K49Q/A174S of isoenzyme GLN1,4 Arabidopsis thaliana
4.18
-
 L-Glu isoenzyme GLN1,4 Arabidopsis thaliana
4.96
-
 L-Glu mutant K49Q of isoenzyme GLN1,4 Arabidopsis thaliana
5.33
-
 NH4+ mutant K49Q of isoenzyme GLN1,4 Arabidopsis thaliana
6.19
-
 NH4+ mutant A174S of isoenzyme GLN1,3 Arabidopsis thaliana
6.59
-
 L-Glu mutant K49Q of isoenzyme GLN1,3 Arabidopsis thaliana
6.83
-
 NH4+ mutant K49Q/A174S of isoenzyme GLN1,3 Arabidopsis thaliana
7.02
-
 L-Glu isoenzyme GLN1,3 Arabidopsis thaliana
7.1
-
 NH4+ isoenzyme GLN1,3 Arabidopsis thaliana
7.23
-
 NH4+ mutant K49Q of isoenzyme GLN1,3 Arabidopsis thaliana
7.97
-
 L-Glu mutant A174S of isoenzyme GLN1,3 Arabidopsis thaliana
8.08
-
 L-Glu mutant K49Q/A174S of isoenzyme GLN1,3 Arabidopsis thaliana