Protein Variants | Comment | Organism |
---|---|---|
S186F | kinetic properties are similar to the wild-type protein, mutant S186F enzyme is resistant to feedback inhibition by glutamine and AMP. S186F protein has a lower affinity for glutamine and AMP than the wild-type enzyme. S186F glutamine synthetase is defective in its ability to block DNA binding by TnrA in vitro | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | mutant S186F enzyme is resistant to feedback inhibition by glutamine and AMP | Bacillus subtilis | |
glutamine | feed-back inhibition of wild-type enzyme, mutant S186F enzyme is resistant to feedback inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
ATP | wild-type enzyme | Bacillus subtilis | |
2.5 | - |
ATP | mutant enzyme S186F | Bacillus subtilis | |
2.9 | - |
hydroxylamine | wild-type enzyme | Bacillus subtilis | |
3.4 | - |
hydroxylamine | mutant enzyme S186F | Bacillus subtilis | |
13 | - |
L-glutamine | wild-type enzyme | Bacillus subtilis | |
14 | - |
L-glutamine | mutant enzyme S186F | Bacillus subtilis | |
27 | - |
L-glutamate | wild-type enzyme | Bacillus subtilis | |
29 | - |
L-glutamate | mutant enzyme S186F | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Bacillus subtilis | |
Mn2+ | activates | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
wild-type enzyme | Bacillus subtilis |
2.7 | - |
mutant enzyme S186F | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH4+ | - |
Bacillus subtilis | ADP + phosphate + L-glutamine | - |
? | |
L-glutamine + hydroxylamine + ADP | - |
Bacillus subtilis | gamma-glutamylhydroxamate + NH4+ + ? | - |
? |