Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.1.2 extracted from

  • Wray, L.V., Jr.; Fisher, S.H.
    A feedback-resistant mutant of Bacillus subtilis glutamine synthetase with pleiotropic defects in nitrogen-regulated gene expression (2005), J. Biol. Chem., 280, 33298-33304.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
S186F kinetic properties are similar to the wild-type protein, mutant S186F enzyme is resistant to feedback inhibition by glutamine and AMP. S186F protein has a lower affinity for glutamine and AMP than the wild-type enzyme. S186F glutamine synthetase is defective in its ability to block DNA binding by TnrA in vitro Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
ATP mutant S186F enzyme is resistant to feedback inhibition by glutamine and AMP Bacillus subtilis
glutamine feed-back inhibition of wild-type enzyme, mutant S186F enzyme is resistant to feedback inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
ATP wild-type enzyme Bacillus subtilis
2.5
-
ATP mutant enzyme S186F Bacillus subtilis
2.9
-
hydroxylamine wild-type enzyme Bacillus subtilis
3.4
-
hydroxylamine mutant enzyme S186F Bacillus subtilis
13
-
L-glutamine wild-type enzyme Bacillus subtilis
14
-
L-glutamine mutant enzyme S186F Bacillus subtilis
27
-
L-glutamate wild-type enzyme Bacillus subtilis
29
-
L-glutamate mutant enzyme S186F Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Bacillus subtilis
Mn2+ activates Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
wild-type enzyme Bacillus subtilis
2.7
-
mutant enzyme S186F Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH4+
-
Bacillus subtilis ADP + phosphate + L-glutamine
-
?
L-glutamine + hydroxylamine + ADP
-
Bacillus subtilis gamma-glutamylhydroxamate + NH4+ + ?
-
?