Cloned (Comment) | Organism |
---|---|
expression of isoenzyme GLN1,1 in Escherichia coli | Arabidopsis thaliana |
expression of isoenzyme GLN1,2 in Escherichia coli | Arabidopsis thaliana |
expression of isoenzyme GLN1,3 in Escherichia coli | Arabidopsis thaliana |
expression of isoenzyme GLN1,4 in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamate | at high concentrations, isoenzyme GLN1,3 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NH4+ | pH 7.8, 30°C, below, isoenzyme GLN1,1 | Arabidopsis thaliana | |
0.3 | - |
ATP | pH 7.8, 30°C, isoenzyme GLN1,1 | Arabidopsis thaliana | |
0.56 | - |
ATP | pH 7.8, 30°C, isoenzyme GLN1,4 | Arabidopsis thaliana | |
0.58 | - |
NH4+ | pH 7.8, 30°C, below, isoenzyme GLN1,4 | Arabidopsis thaliana | |
0.6 | - |
L-glutamate | pH 7.8, 30°C, isoenzyme GLN1,4 | Arabidopsis thaliana | |
0.85 | - |
ATP | pH 7.8, 30°C, isoenzyme GLN1,3 | Arabidopsis thaliana | |
1.1 | - |
L-glutamate | pH 7.8, 30°C, isoenzyme GLN1,1 | Arabidopsis thaliana | |
1.1 | - |
ATP | pH 7.8, 30°C, isoenzyme GLN1,2 | Arabidopsis thaliana | |
1.21 | - |
NH4+ | pH 7.8, 30°C, below, isoenzyme GLN1,3 | Arabidopsis thaliana | |
2.45 | - |
NH4+ | pH 7.8, 30°C, below, isoenzyme GLN1,2 | Arabidopsis thaliana | |
3.8 | - |
L-glutamate | pH 7.8, 30°C, isoenzyme GLN1,2 | Arabidopsis thaliana | |
3.9 | - |
L-glutamate | pH 7.8, 30°C, isoenzyme GLN1,3 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH4+ | Arabidopsis thaliana | glutamine synthetase is a key enzyme in nitrogen assimilation | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH4+ | Arabidopsis thaliana | glutamine synthetase is a key enzyme in nitrogen assimilation. Isoenzyme GLN1,1 accumulates in the surface layers of root during nitrogen limitation | ADP + phosphate + L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoenzyme GLN1,2 | - |
Arabidopsis thaliana | Q56WN1 | isoenzyme GLN1,1 | - |
Arabidopsis thaliana | Q9FMD9 | isoenzyme GLN1,4 | - |
Arabidopsis thaliana | Q9LVI8 | isoenzyme GLN1,3 | - |
Purification (Comment) | Organism |
---|---|
recombinant isoenzyme GLN1,1 | Arabidopsis thaliana |
recombinant isoenzyme GLN1,2 | Arabidopsis thaliana |
recombinant isoenzyme GLN1,3 | Arabidopsis thaliana |
recombinant isoenzyme GLN1,4 | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | isoenzyme GLN1,1 | Arabidopsis thaliana | - |
leaf | isoenzyme GLN1,2 | Arabidopsis thaliana | - |
leaf | isoenzyme GLN1,3 | Arabidopsis thaliana | - |
additional information | expressed in nitrogen-starved plants, but 10fold less abundant than isoenzyme GLN1,1 | Arabidopsis thaliana | - |
root | isoenzyme GLN1,1 accumulates in the surface layers of root during nitrogen limitation | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH4+ | glutamine synthetase is a key enzyme in nitrogen assimilation | Arabidopsis thaliana | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH4+ | glutamine synthetase is a key enzyme in nitrogen assimilation. Isoenzyme GLN1,1 accumulates in the surface layers of root during nitrogen limitation | Arabidopsis thaliana | ADP + phosphate + L-glutamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLN1,1 | - |
Arabidopsis thaliana |
GLN1,2 | - |
Arabidopsis thaliana |
GLN1,3 | - |
Arabidopsis thaliana |
GLN1,4 | - |
Arabidopsis thaliana |