Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | enzyme form GSIII | Sinorhizobium meliloti | |
diphosphate | enzyme form GSIII | Sinorhizobium meliloti | |
L-methionine sulfoximine | the concentration needed to inhibit GSIII is 50-100times higher than that needed to inhibit GSI or GSII | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.3 | - |
hydroxylamine | enzyme form GSIII | Sinorhizobium meliloti | |
13.3 | - |
Glu | enzyme form GSIII | Sinorhizobium meliloti | |
33 | - |
NH4+ | enzyme form GSIII | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46500 | - |
x * 46500 + x * 49000, SDS-PAGE | Sinorhizobium meliloti |
49000 | - |
x * 46500 + x * 49000, SDS-PAGE | Sinorhizobium meliloti |
355000 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
3 enzyme forms: GSI, GSII and GSIII | - |
Purification (Comment) | Organism |
---|---|
GSIII | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Sinorhizobium meliloti | ADP + phosphate + L-Gln | - |
? | |
ATP + L-glutamate + hydroxylamine | - |
Sinorhizobium meliloti | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | x * 46500 + x * 49000, SDS-PAGE | Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme form GSIII | Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
enzyme form GSIII | Sinorhizobium meliloti |