KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
[Bacillus] caldolyticus | |
0.2 | - |
NH4+ | NH4+, biosynthetic assay, Mg2+-activated, enzyme form EII | [Bacillus] caldolyticus | |
0.35 | - |
hydroxylamine | Mn2+-activated, enzyme form EII | [Bacillus] caldolyticus | |
0.4 | - |
hydroxylamine | Mn2+-activated, enzyme form EI | [Bacillus] caldolyticus | |
0.48 | - |
NH4+ | biosynthetic assay, Mn2+-stimulated, enzyme form EI | [Bacillus] caldolyticus | |
0.53 | - |
ATP | biosynthetic assay, Mg2+-activated, enzyme form EII | [Bacillus] caldolyticus | |
0.56 | - |
NH4+ | hydroxylamine, Mg2+-activated, enzyme form EII | [Bacillus] caldolyticus | |
1.5 | - |
ATP | ATP, biosynthetic assay, Mn2+-activated, enzyme form EII | [Bacillus] caldolyticus | |
2.9 | - |
ATP | biosynthetic assay, Mg2+-activated, enzyme form EI | [Bacillus] caldolyticus | |
3 | - |
Glu | ATP, biosynthetic assay, Mn2+-activated, enzyme form EI | [Bacillus] caldolyticus | |
17 | - |
L-Glu | biosynthetic assay, Mg2+-activated, enzyme form I or Mn2+-activated, enzyme form EII. | [Bacillus] caldolyticus | |
18 | - |
L-Glu | L-Glu, biosynthetic assay, Mg2+-activated, enzyme form EII | [Bacillus] caldolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates enzyme form EII more than EI | [Bacillus] caldolyticus | |
Co2+ | only enzyme form E1 is activated | [Bacillus] caldolyticus | |
Mg2+ | divalent cation required, enzyme form EI is more active with Mg2+ than with Mn2+, but EII is more active with Mn2+ than Mg2+ | [Bacillus] caldolyticus | |
Mn2+ | divalent cation required, enzyme form EI is more active with Mg2+ than with Mn2+, but EII is more active with Mn2+ than Mg2+ | [Bacillus] caldolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
12 * 51000, SDS-PAGE | [Bacillus] caldolyticus |
330000 | 370000 | gel filtration | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldolyticus | - |
two enzyme forms: EI and EII | - |
Purification (Comment) | Organism |
---|---|
two enzyme forms EI and EII | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | enzyme form EII is almost absolutely specific for ATP, but enzyme form E1 can also use ITP, GTP and UTP | [Bacillus] caldolyticus | ADP + phosphate + L-Gln | - |
? | |
Gln + hydroxylamine + ADP | - |
[Bacillus] caldolyticus | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
GTP + L-Glu + NH4+ | enzyme form EII is almost absolutely specific for ATP, but enzyme form E1 can also use ITP, GTP and UTP | [Bacillus] caldolyticus | GDP + phosphate + L-Gln | - |
? | |
ITP + L-Glu + NH4+ | enzyme form EII is almost absolutely specific for ATP, but E1 can also use ITP, GTP and UTP | [Bacillus] caldolyticus | IDP + phosphate + L-Gln | - |
? | |
UTP + L-Glu + NH4+ | enzyme form EII is almost absolutely specific for ATP, but E1 can also use ITP, GTP and UTP | [Bacillus] caldolyticus | UDP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 51000, SDS-PAGE | [Bacillus] caldolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
thermal denaturation above, even with substrates bound | [Bacillus] caldolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | Mn2+-stimulated activity, enzyme forms EI and EII, with a ratio of Mn2+ to ATP of 1.0 | [Bacillus] caldolyticus |
7.5 | - |
Mg2+-stimulated enzyme, at a ratio of Mg2+ to ATP of above 2 | [Bacillus] caldolyticus |