Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethylene glycol | 4-8%, 50% activation | Squalus acanthias |
General Stability | Organism |
---|---|
stability is enhanced by the presence of ethylene glycol | Squalus acanthias |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits in presence of Mg2+ | Squalus acanthias | |
F- | - |
Squalus acanthias | |
Urea | physiological concentrations of urea inhibit, at least when ATP and/or glutamate are nonsaturating. Inhibition is partially reversed by trimethylamine-N-oxide | Squalus acanthias |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Squalus acanthias | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ in excess of that required for formation of MgATP2- is required for maximal activity | Squalus acanthias | |
Mn2+ | cannot effectively replace Mg2+ | Squalus acanthias |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Squalus acanthias |
200000 | - |
gel filtration, in presence of Mg2+, MgATP2-, and L-Glu the enzyme has a MW of 400000, reversible dissociation from the 400000 MW form to a species of MW 200000 in the absence of MgATP2- and L-Glu | Squalus acanthias |
400000 | - |
gel filtration, in presence of Mg2+, MgATP2-, and L-Glu, reversible dissociation to a species of MW 200000 in the absence of MgATP2- and L-Glu | Squalus acanthias |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | Squalus acanthias | first step in urea synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Squalus acanthias | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55.8 | - |
- |
Squalus acanthias |
Storage Stability | Organism |
---|---|
-20°C, 10 mM MgSO4, 4 mM ATP, 50 mM glutamate, 100 mM NaCl, 10% ethylene glycol, 20 mM Hepes buffer, pH 7.4, less than 5% loss of activity per week | Squalus acanthias |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Squalus acanthias | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | first step in urea synthesis | Squalus acanthias | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Squalus acanthias |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.4 | - |
Squalus acanthias |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | half-maximal activity at pH 6.5 and 8.0 | Squalus acanthias |