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Literature summary for 6.3.1.2 extracted from
- Glutamine synthetase of pea leaf and seed cytosol. Structure and properties (1985), Biochim. Biophys. Acta, 828, 336-350.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
NH4+ | - |
Pisum sativum |  |
| 3 | - |
Glu | - |
Pisum sativum | |
| 14 | - |
Glu | - |
Pisum sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Pisum sativum | |
| Mn2+ | activates | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
8 * 48000, isoenzyme GScs, SDS-PAGE | Pisum sativum |
| 64000 | - |
8 * 64000, isoenzyme GScl, SDS-PAGE | Pisum sativum |
| 384000 | - |
isoenzyme GScs, gel filtration | Pisum sativum |
| 520000 | - |
isoenzyme GScl, gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
isoenzymes GScs from seed cytosol and isoenzyme GScl from leaf cytosol | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes GScs from seed cytosol and isoenzyme GScl from leaf cytosol | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
| seed | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + NH4+ | - |
Pisum sativum | ADP + phosphate + L-Gln | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 48000, isoenzyme GScs, SDS-PAGE | Pisum sativum |
| octamer | 8 * 64000, isoenzyme GScl, SDS-PAGE | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
isoenzyme GScl, Mn2+-activated enzyme | Pisum sativum |
| 5.5 | - |
isoenzyme GScs, Mn2+-activated enzyme | Pisum sativum |
| 7.2 | - |
isoenzyme GScs, Mg2+-activated enzyme | Pisum sativum |
| 7.5 | - |
isoenzyme GScl, Mg2+-activated enzyme | Pisum sativum |
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