Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala | strong inhibition of transferase activity | Rhodobacter capsulatus | |
Asp | inhibition of biosynthetic and transferase activity | Rhodobacter capsulatus | |
Gly | inhibition of biosynthetic and transferase activity | Rhodobacter capsulatus | |
Ser | inhibition of biosynthetic and transferase activity | Rhodobacter capsulatus | |
Val | inhibition of biosynthetic and transferase activity | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ADP | transferase activity | Rhodobacter capsulatus | |
6 | - |
hydroxylamine | transferase activity | Rhodobacter capsulatus | |
12 | - |
L-Gln | transferase activity | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
12 * 55000, the enzyme exhibits different aggregation states with detectable oligomers of 1, 2, 3, 4, 6, 8, 10, and 12 subunits, SDS-PAGE | Rhodobacter capsulatus |
660000 | 670000 | disc gel electrophoresis, pore gradient electrophoresis, sucrose density gradient centrifugation | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Rhodobacter capsulatus E1F1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
153 | - |
gamma-glutamyl transferase assay | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Rhodobacter capsulatus | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Rhodobacter capsulatus E1F1 | ADP + phosphate + L-Gln | - |
? | |
Gln + hydroxylamine + ADP | - |
Rhodobacter capsulatus | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Gln + hydroxylamine + ADP | - |
Rhodobacter capsulatus E1F1 | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 55000, the enzyme exhibits different aggregation states with detectable oligomers of 1, 2, 3, 4, 6, 8, 10, and 12 subunits, SDS-PAGE | Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover number 4200, biosynthetic activity. 102000, transferase activity | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
adenylylated form, n:9, transferase activity | Rhodobacter capsulatus |
7 | - |
intermediate form, n:6, transferase activity | Rhodobacter capsulatus |
7.5 | - |
deadenylylated form, transferase activity | Rhodobacter capsulatus |