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Literature summary for 6.3.1.2 extracted from

  • Krishnan, I.S.; Singhal, R.K.; Dua, R.D.
    Purification and characterization of glutamine synthetase from Clostridium pasteurianum (1986), Biochemistry, 25, 1589-1599.
    View publication on PubMed

General Stability

General Stability Organism
purified enzyme being very unstable, is stabilized by 25% v/v glycerol Clostridium pasteurianum
substrates, as L-Glu, Mg2+, and MgATP2- protect synthetase and glutamyl transferase activity against thermal inactivation Clostridium pasteurianum

Inhibitors

Inhibitors Comment Organism Structure
Ala
-
Clostridium pasteurianum
Asp
-
Clostridium pasteurianum
Gly
-
Clostridium pasteurianum
Ser
-
Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
ATP
-
Clostridium pasteurianum
2.5
-
ATP arsenate Clostridium pasteurianum
3.4
-
L-Glu synthetase reaction Clostridium pasteurianum
3.8
-
ADP
-
Clostridium pasteurianum
4.1
-
hydroxylamine transferase reaction Clostridium pasteurianum
14.7
-
Gln
-
Clostridium pasteurianum
22.2
-
L-Gln transferase reaction Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at a ratio of Mg2+ to ATP of 2 Clostridium pasteurianum
Mn2+ maximal synthetase activity occurs when the ratio of Mg2+ to ATP is 2 Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
20 * 50000, SDS-PAGE Clostridium pasteurianum
1000000
-
gel filtration Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Clostridium pasteurianum

Storage Stability

Storage Stability Organism
at 4°C or in the frozen state, 200 mM potassium phosphate buffer, pH 7.2, 10 mM 2-ketoglutarate, 5 mM 2-mercaptoethanol, 1 mM EDTA, total loss of activity after 24 h, in presence of 25%-50% glycerol 80-90% retention of activity after 2 months Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + hydroxylamine
-
Clostridium pasteurianum ADP + phosphate + gamma-glutamyl hydroxamate
-
?
ATP + L-Glu + NH4+
-
Clostridium pasteurianum ADP + phosphate + L-Gln
-
?
Gln + hydroxylamine + ADP
-
Clostridium pasteurianum gamma-Glutamylhydroxamate + NH4+ + ?
-
?

Subunits

Subunits Comment Organism
eicosamer 20 * 50000, SDS-PAGE Clostridium pasteurianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
synthetase and gamma-glutamyl transferase activity Clostridium pasteurianum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 40% of maximal activity, 60°C: about 65% of maximal activity, glutamyl transferase activity Clostridium pasteurianum
40 60 40°C: about 45% of maximal activity, 60°C: about 75% of maximal activity, glutamyl transferase activity Clostridium pasteurianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
substrates, as L-Glu, Mg2+, and MgATP2- protect synthetase and glutamyl transferase activity against thermal inactivation Clostridium pasteurianum
50
-
half-life: 8 min, synthetase and glutamyl transferase activity Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
gamma-glutamyl transferase activity and with ATP + L-Gln + hydroxylamine as substrates Clostridium pasteurianum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 60% of maximal activity, pH 8.0: about 55% of maximal activity, glutamyl transferase activity Clostridium pasteurianum
6 9 Ph 6.0: about 30% of maximal activity, pH 9.0: about 20% of maximal activity, ATP + L-Glu + hydroxylamine Clostridium pasteurianum