General Stability | Organism |
---|---|
purified enzyme being very unstable, is stabilized by 25% v/v glycerol | Clostridium pasteurianum |
substrates, as L-Glu, Mg2+, and MgATP2- protect synthetase and glutamyl transferase activity against thermal inactivation | Clostridium pasteurianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala | - |
Clostridium pasteurianum | |
Asp | - |
Clostridium pasteurianum | |
Gly | - |
Clostridium pasteurianum | |
Ser | - |
Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
ATP | - |
Clostridium pasteurianum | |
2.5 | - |
ATP | arsenate | Clostridium pasteurianum | |
3.4 | - |
L-Glu | synthetase reaction | Clostridium pasteurianum | |
3.8 | - |
ADP | - |
Clostridium pasteurianum | |
4.1 | - |
hydroxylamine | transferase reaction | Clostridium pasteurianum | |
14.7 | - |
Gln | - |
Clostridium pasteurianum | |
22.2 | - |
L-Gln | transferase reaction | Clostridium pasteurianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at a ratio of Mg2+ to ATP of 2 | Clostridium pasteurianum | |
Mn2+ | maximal synthetase activity occurs when the ratio of Mg2+ to ATP is 2 | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
20 * 50000, SDS-PAGE | Clostridium pasteurianum |
1000000 | - |
gel filtration | Clostridium pasteurianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium pasteurianum |
Storage Stability | Organism |
---|---|
at 4°C or in the frozen state, 200 mM potassium phosphate buffer, pH 7.2, 10 mM 2-ketoglutarate, 5 mM 2-mercaptoethanol, 1 mM EDTA, total loss of activity after 24 h, in presence of 25%-50% glycerol 80-90% retention of activity after 2 months | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + hydroxylamine | - |
Clostridium pasteurianum | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + NH4+ | - |
Clostridium pasteurianum | ADP + phosphate + L-Gln | - |
? | |
Gln + hydroxylamine + ADP | - |
Clostridium pasteurianum | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
eicosamer | 20 * 50000, SDS-PAGE | Clostridium pasteurianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
synthetase and gamma-glutamyl transferase activity | Clostridium pasteurianum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 40% of maximal activity, 60°C: about 65% of maximal activity, glutamyl transferase activity | Clostridium pasteurianum |
40 | 60 | 40°C: about 45% of maximal activity, 60°C: about 75% of maximal activity, glutamyl transferase activity | Clostridium pasteurianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
substrates, as L-Glu, Mg2+, and MgATP2- protect synthetase and glutamyl transferase activity against thermal inactivation | Clostridium pasteurianum |
50 | - |
half-life: 8 min, synthetase and glutamyl transferase activity | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
gamma-glutamyl transferase activity and with ATP + L-Gln + hydroxylamine as substrates | Clostridium pasteurianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 60% of maximal activity, pH 8.0: about 55% of maximal activity, glutamyl transferase activity | Clostridium pasteurianum |
6 | 9 | Ph 6.0: about 30% of maximal activity, pH 9.0: about 20% of maximal activity, ATP + L-Glu + hydroxylamine | Clostridium pasteurianum |