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Literature summary for 6.3.1.2 extracted from

  • Kumar, P.S.; Rao, S.L.N.
    Identification and characterization of three forms of glutamine synthetase unique to Rhizobia (1986), Curr. Microbiol., 14, 113-116.
No PubMed abstract available
Search for more literature for 6.3.1.2

Inhibitors

Inhibitors Comment Organism Structure
Ala beta-Ala; inhibition of enzyme form GSII and GSIII Rhizobium phaseoli
L-methionine sulfoximine inhibition of enzyme form GSII and GSIII Rhizobium phaseoli
L-methionine sulfoximine
-
 Sinorhizobium meliloti
Mg2+ inhibits enzyme form GSIII to nearly 72%, enzyme form GSI 30%, and enzyme form GSII 33.3% Rhizobium phaseoli
thymidine complete inhibition of enzyme form GSII, partial inhibition of enzyme form GSI, no effect on enzyme form GSIII Rhizobium phaseoli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64570
-
 enzyme form GSI, gel filtration Rhizobium phaseoli
489800
-
 enzyme form GSII, gel filtration Rhizobium phaseoli
891000
-
 enzyme form GSIII, gel filtration Rhizobium phaseoli

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum
-
 3 enzyme forms: GSI, GSII and GSIII
-
Rhizobium phaseoli
-
-
-
Sinorhizobium meliloti
-
 3 enzyme forms: GSI, GSII and GSIII
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Sinorhizobium meliloti ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Rhizobium leguminosarum ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Rhizobium phaseoli ADP + phosphate + L-Gln
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 20.5% inactivation of enzyme form GSI, 55.4% inactivation of enzyme form GSII, 27.3% inactivation of enzyme form GSIII Rhizobium phaseoli
60
-
 15 min, 40% inactivation of enzyme form GSI, 74.6% inactivation of enzyme form GSII, 35.5% inactivation of enzyme form GSIII Rhizobium leguminosarum
60
-
 15 min, 45.8% inactivation of enzyme form GSI, 61.2% inactivation of enzyme form GSII, 37.5% inactivation of enzyme form GSIII Sinorhizobium meliloti