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Literature summary for 6.3.1.13 extracted from
- Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC) (2007), Biochemistry, 46, 11421-11429.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli strain BL21 | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-O-[N-(L-cysteinyl)sulfamonyl]adenosine | a stable bisubstrate analogue, exhibits competitive inhibition versus ATP and noncompetitive inhibition versus cysteine | Mycolicibacterium smegmatis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state and pre-steady-state kinetic analysis, single-turnover reactions of the first and second half reactions | Mycolicibacterium smegmatis | |
| 0.1 | - |
L-cysteine | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis | |
| 0.16 | - |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis | |
| 1.8 | - |
ATP | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40700 | - |
recombinant His-tagged enzyme, gel filtration | Mycolicibacterium smegmatis |
| 47000 | - |
1 * 47000, recombinant His-tagged enzyme, 1 * 47562, amino acid sequence calculation | Mycolicibacterium smegmatis |
| 47562 | - |
1 * 47000, recombinant His-tagged enzyme, 1 * 47562, amino acid sequence calculation | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis | a key step in mycothiol, i.e. 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-R-D-glucopyranoside or MSH or AcCys-GlcN-Ins, biosynthesis, mycothiol is produced to act against oxidative and antibiotic stress and is essential for cell growth, biosynthetic pathway, overview | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration to about 95% purity | Mycolicibacterium smegmatis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | Bi Uni Uni Bi Ping Pong reaction mechanism | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | a key step in mycothiol, i.e. 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-R-D-glucopyranoside or MSH or AcCys-GlcN-Ins, biosynthesis, mycothiol is produced to act against oxidative and antibiotic stress and is essential for cell growth, biosynthetic pathway, overview | Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, recombinant His-tagged enzyme, 1 * 47562, amino acid sequence calculation | Mycolicibacterium smegmatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cysteine ligase | - |
Mycolicibacterium smegmatis |
| MshC | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycolicibacterium smegmatis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.15 | - |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mycolicibacterium smegmatis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00031 | - |
5'-O-[N-(L-cysteinyl)sulfamonyl]adenosine | versus ATP | Mycolicibacterium smegmatis | |
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