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Literature summary for 6.3.1.13 extracted from
- ATP-dependent L-cysteine:1D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, mycothiol biosynthesis enzyme MshC, is related to class I cysteinyl-tRNA synthetases (2002), Biochemistry, 41, 6885-6890.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | enzyme is a traget for drug development against actinomycetes | Mycolicibacterium smegmatis |
| pharmacology | enzyme is a traget for drug development against actinomycetes | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme | Mycobacterium tuberculosis |
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as N-terminally His-tagged enzyme | Mycolicibacterium smegmatis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
L-cysteine | pH 7.5, 37°C | Mycolicibacterium smegmatis |  |
| 0.072 | - |
1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycobacterium tuberculosis | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis mc(2)155 | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
gene mshC, formerly gene cys2 | - |
| Mycolicibacterium smegmatis | - |
gene mshC, formerly gene cys2, 2 different splicing variants due to a second start codon | - |
| Mycolicibacterium smegmatis mc(2)155 | - |
gene mshC, formerly gene cys2, 2 different splicing variants due to a second start codon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2400fold, 2 peaks | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.054 | - |
purified enzyme | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis mc(2)155 | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
| 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | Mycolicibacterium smegmatis mc(2)155 | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cys:GlcN-Ins ligase | - |
Mycolicibacterium smegmatis |
| Cys:GlcN-Ins ligase | - |
Mycobacterium tuberculosis |
| More | enzyme is related to class I cysteinyl-tRNA synthetase | Mycolicibacterium smegmatis |
| More | enzyme is related to class I cysteinyl-tRNA synthetase | Mycobacterium tuberculosis |
| MshC ligase | - |
Mycolicibacterium smegmatis |
| MshC ligase | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Mycolicibacterium smegmatis | |
| ATP | dependent on | Mycobacterium tuberculosis | |
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