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Literature summary for 6.2.1.3 extracted from
- Discovery of amide (peptide) bond synthetic activity in acyl-CoA synthetase (2008), J. Biol. Chem., 283, 11312-11321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene acsA, expression of His-tagged enzyme | Pseudomonas chlororaphis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas chlororaphis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas chlororaphis | Q5CD72 | gene acsA | - |
| Pseudomonas chlororaphis B23 | Q5CD72 | gene acsA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged AcsA by nickel affinity chromatography | Pseudomonas chlororaphis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also forms a carbon-nitrogen bond, reaction of EC 6.3.1 acid-ammonia (or amide) ligase, i.e. amide synthase, and EC 6.3.2 acid-amino acid ligase, i.e. peptide synthase, comprising the amino group of the cysteine and the carboxyl group of the acid, overview | Pseudomonas chlororaphis | ? | - |
? | |
| additional information | the enzyme also forms a carbon-nitrogen bond, reaction of EC 6.3.1 acid-ammonia (or amide) ligase, i.e. amide synthase, and EC 6.3.2 acid-amino acid ligase, i.e. peptide synthase, comprising the amino group of the cysteine and the carboxyl group of the acid, overview | Pseudomonas chlororaphis B23 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcsA | - |
Pseudomonas chlororaphis |
| Acyl-CoA synthetase | - |
Pseudomonas chlororaphis |
| More | acyl-CoA synthetase belongs to the superfamily of adenylate-forming enzymes, whose three-dimensional structures are analogous to one another | Pseudomonas chlororaphis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Pseudomonas chlororaphis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas chlororaphis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pseudomonas chlororaphis | |
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Release 2023.1 (January 2023)
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