Cloned (Comment) | Organism |
---|---|
expression of ACS-Flag fusion proteins in Escherichia coli | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cis-9,10-methylene octadecanoic acid | IC50: 0.025 mM for ACS1-Flag fusion protein, 0.03-0.04 mM for ACS4-Flag fusion protein, no effect on ACS5-Flag fusion protein | Rattus norvegicus | |
GW1929 | IC50: above 0.05 mM for ACS1-Flag fusion protein, 0.05 mM for ACS4-Flag fusion protein, no effect on ACS5-Flag fusion protein | Rattus norvegicus | |
additional information | 3-phenoxybenzoic acid, p-coumaric acid, ibuprofen, ferulic acid and firefly luciferin, at concentrations up to 0.05 mM have no effect on three ACS isoenzymes | Rattus norvegicus | |
NEM | strongly inhibits ACS5 and weakly inhibits ACS1, no effect on ACS5 | Rattus norvegicus | |
pioglitazone | IC50: 0.0015 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein | Rattus norvegicus | |
rosiglitazone | IC50: 0.0005 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein | Rattus norvegicus | |
thiazolidinediones | specific inhibitors of ACS4 | Rattus norvegicus | |
Triacsin C | strongly inhibits ACS1 and ACS4, no effect on ACS5 | Rattus norvegicus | |
troglitazone | IC50: 0.0015 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
CoA | pH 7.5, 37°C, ACS5 | Rattus norvegicus | |
0.0041 | - |
CoA | pH 7.5, 37°C, ACS4 | Rattus norvegicus | |
0.005 | - |
palmitate | pH 7.5, 37°C, ACS1 | Rattus norvegicus | |
0.0054 | - |
palmitate | pH 7.5, 37°C, ACS4 | Rattus norvegicus | |
0.0064 | - |
CoA | pH 7.5, 37°C, ACS1 | Rattus norvegicus | |
0.0086 | - |
palmitate | pH 7.5, 37°C, ACS5 | Rattus norvegicus | |
0.034 | - |
ATP | pH 7.5, 37°C, ACS4 | Rattus norvegicus | |
0.649 | - |
ATP | pH 7.5, 37°C, ACS1 | Rattus norvegicus | |
0.666 | - |
ATP | pH 7.5, 37°C, ACS5 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + palmitate + CoA | - |
Rattus norvegicus | AMP + diphosphate + palmitoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACS1 | - |
Rattus norvegicus |
ACS4 | - |
Rattus norvegicus |
ACS5 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7.4 | 9 | ACS1 and ACS4 | Rattus norvegicus |
7.4 | 8 | ACS5 | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
half-life: about 10 min for ACS1, shorter thah 1 min fpr ACS4 and about 4 min for ACS5 | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0005 | - |
IC50: 0.0005 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein | Rattus norvegicus | rosiglitazone | |
0.0015 | - |
IC50: 0.0015 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein | Rattus norvegicus | troglitazone | |
0.0015 | - |
IC50: 0.0015 mM for ACS1-Flag fusion protein, no effect on ACS4-Flag fusion protein and ACS5-Flag fusion protein | Rattus norvegicus | pioglitazone | |
0.025 | - |
IC50: 0.025 mM for ACS1-Flag fusion protein, 0.03-0.04 mM for ACS4-Flag fusion protein, no effect on ACS5-Flag fusion protein | Rattus norvegicus | cis-9,10-methylene octadecanoic acid | |
0.05 | - |
IC50: above 0.05 mM for ACS1-Flag fusion protein, 0.05 mM for ACS4-Flag fusion protein, no effect on ACS5-Flag fusion protein | Rattus norvegicus | GW1929 |