Cloned (Comment) | Organism |
---|---|
gene AAE14, complementates an Escherichia coli mutant disrupted in the menE gene encoding the enzyme, and restores menaquinone biosynthesis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of three aae14 mutant alleles by reverse genetics, that are seedling lethal, and show no detectable phylloquinon, phenotype, overview. Weak expression of an AAE14 transgene in mutant Arabidopsis thaliana plants, controlled by the uninduced XVE promoter, results in chlorotic, slow-growing plants that accumulate phylloquinone. Inducing the XVE promoter in these plants, or expressing an AAE14 transgene under the control of the CaMV 35S promoter, led to full complementation of the mutant phenotype, aae14-mutant plants are also able to synthesize phylloquinone when provided with 1,4-dihydroxy-2-naphthoate, an intermediate in phylloquinone synthesis downstream of the OSB-CoA ligase reaction, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast stroma | - |
Arabidopsis thaliana | 9570 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-succinylbenzoate + CoA | Arabidopsis thaliana | OSB-CoA ligase acts in phylloquinone synthesis, pathway for phylloquinone synthesis, overview | AMP + diphosphate + 2-succinylbenzoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VYJ1 | gene AAE14 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-succinylbenzoate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 2-succinylbenzoyl-CoA | - |
? | |
ATP + 2-succinylbenzoate + CoA | OSB-CoA ligase acts in phylloquinone synthesis, pathway for phylloquinone synthesis, overview | Arabidopsis thaliana | AMP + diphosphate + 2-succinylbenzoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAE14 | - |
Arabidopsis thaliana |
acyl-activating enzyme 14 | - |
Arabidopsis thaliana |
o-succinylbenzoyl-coenzyme A ligase | - |
Arabidopsis thaliana |
OSB-CoA ligase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |