Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.26 extracted from

  • Kim, H.U.; van Oostende, C.; Basset, G.J.; Browse, J.
    The AAE14 gene encodes the Arabidopsis o-succinylbenzoyl-CoA ligase that is essential for phylloquinone synthesis and photosystem-I function (2008), Plant J., 54, 272-283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene AAE14, complementates an Escherichia coli mutant disrupted in the menE gene encoding the enzyme, and restores menaquinone biosynthesis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information identification of three aae14 mutant alleles by reverse genetics, that are seedling lethal, and show no detectable phylloquinon, phenotype, overview. Weak expression of an AAE14 transgene in mutant Arabidopsis thaliana plants, controlled by the uninduced XVE promoter, results in chlorotic, slow-growing plants that accumulate phylloquinone. Inducing the XVE promoter in these plants, or expressing an AAE14 transgene under the control of the CaMV 35S promoter, led to full complementation of the mutant phenotype, aae14-mutant plants are also able to synthesize phylloquinone when provided with 1,4-dihydroxy-2-naphthoate, an intermediate in phylloquinone synthesis downstream of the OSB-CoA ligase reaction, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
chloroplast stroma
-
Arabidopsis thaliana 9570
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-succinylbenzoate + CoA Arabidopsis thaliana OSB-CoA ligase acts in phylloquinone synthesis, pathway for phylloquinone synthesis, overview AMP + diphosphate + 2-succinylbenzoyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VYJ1 gene AAE14
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-succinylbenzoate + CoA
-
Arabidopsis thaliana AMP + diphosphate + 2-succinylbenzoyl-CoA
-
?
ATP + 2-succinylbenzoate + CoA OSB-CoA ligase acts in phylloquinone synthesis, pathway for phylloquinone synthesis, overview Arabidopsis thaliana AMP + diphosphate + 2-succinylbenzoyl-CoA
-
?

Synonyms

Synonyms Comment Organism
AAE14
-
Arabidopsis thaliana
acyl-activating enzyme 14
-
Arabidopsis thaliana
o-succinylbenzoyl-coenzyme A ligase
-
Arabidopsis thaliana
OSB-CoA ligase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana