Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no evidence for a phospholipid requirement | Escherichia coli |
General Stability | Organism |
---|---|
ATP protects against heat inactivation at 55°C | Escherichia coli |
Mg2+ is not required for ATP to protect the enzyme from heat denaturation | Escherichia coli |
salts destabilize the enzyme, which results in increased heat lability | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
acyl-carrier protein | - |
Escherichia coli | |
0.0053 | - |
palmitic acid | - |
Escherichia coli | |
0.03 | - |
oleic acid | - |
Escherichia coli | |
0.3 | - |
ATP | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Escherichia coli | - |
- |
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Salt | requirement for high concentrations of salt, with the exception of Mg2+, the requirement for ion is nonspecific. Chaotrophic salts are effective at lower concentrations than less chaotrophic salts | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oleic acid + [acyl-carrier protein] | - |
Escherichia coli | AMP + diphosphate + oleoyl-[acyl-carrier protein] | - |
? | |
ATP + palmitic acid + [acyl-carrier protein] | - |
Escherichia coli | AMP + diphosphate + palmitoyl-[acyl-carrier protein] | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life: 2 min, in presence of ATP the half-life increases to above 32 min | Escherichia coli |