Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | substrate inhibition at high concentrations, above 20 mM for the liver enzyme and above 10 mM for the mammary gland enzyme | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
CoA | cow liver enzyme | Bos taurus | |
1 | - |
CoA | mammary gland enzyme, cow | Bos taurus | |
1.1 | - |
ATP | mammary gland enzyme, cow | Bos taurus | |
1.3 | - |
propanoate | mamary gland enzyme, cow, | Bos taurus | |
4.8 | - |
ATP | cow liver enzyme | Bos taurus | |
5.3 | - |
propanoate | cow liver enzyme | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus | |
Mg2+ | Km: 5.5 mM for cow liver enzyme, Km: 1.6 mM for mammary gland enzyme | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf and midlactation cow | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
mammary gland | of midlactation cow, no activity in calf | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | 22% of the activity relative to propanonate | Bos taurus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + butyrate + CoA | 27% of the activity relative to propanoate | Bos taurus | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + propanoate + CoA | - |
Bos taurus | AMP + diphosphate + propanoyl-CoA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
cow liver enzyme | Bos taurus |
50 | - |
mammary gland enzyme, cow | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
30 | 5 min, maximal stability, cow liver and mammary gland enzymes | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | cow liver enzyme | Bos taurus |
8 | 9 | mammary gland enzyme, cow | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | cow liver enzyme | Bos taurus |
8.5 | 9 | mammary gland enzyme, cow | Bos taurus |