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Literature summary for 6.2.1.1 extracted from

  • Ingram-Smith, C.; Thurman, J.L.; Zimowski, K.; Smith, K.S.
    Role of motif III in catalysis by acetyl-CoA synthetase (2012), Archaea, 2012, 509579.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
A498A alteration does not significantly affect the Km for any substrate but reduces the turnover rate kcat 41fold Methanothermobacter thermautotrophicus
A500T kcat value decreases 44fold Methanothermobacter thermautotrophicus
D502A inactive mutant enzyme Methanothermobacter thermautotrophicus
D502E inactive mutant enzyme Methanothermobacter thermautotrophicus
D502N inactive mutant enzyme Methanothermobacter thermautotrophicus
G501A two- to threefold reduced Km values for acetate, ATP and CoA. The turnover rate is over 200fold reduced Methanothermobacter thermautotrophicus
T499A kcat value decreases 83fold Methanothermobacter thermautotrophicus
Y498F inactive mutant enzyme Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
CoA pH 7.0, 65°C, mutant enzyme G501A Methanothermobacter thermautotrophicus
0.1
-
CoA pH 7.0, 65°C, mutant enzyme Y498A Methanothermobacter thermautotrophicus
0.19
-
CoA pH 7.0, 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
0.24
-
CoA pH 7.0, 65°C, mutant enzyme T499A Methanothermobacter thermautotrophicus
0.5 1 CoA pH 7.0, 65°C, mutant enzyme A500T Methanothermobacter thermautotrophicus
1.4
-
acetate pH 7.0, 65°C, mutant enzyme G501A Methanothermobacter thermautotrophicus
1.7
-
ATP pH 7.0, 65°C, mutant enzyme G501A Methanothermobacter thermautotrophicus
1.7
-
ATP pH 7.0, 65°C, mutant enzyme T499A Methanothermobacter thermautotrophicus
1.7
-
ATP pH 7.0, 65°C, mutant enzyme Y498A Methanothermobacter thermautotrophicus
2.1
-
acetate pH 7.0, 65°C, mutant enzyme A500T Methanothermobacter thermautotrophicus
3
-
acetate pH 7.0, 65°C, mutant enzyme T499A Methanothermobacter thermautotrophicus
3.3
-
ATP pH 7.0, 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
3.5
-
acetate pH 7.0, 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
3.6
-
ATP pH 7.0, 65°C, mutant enzyme A500T Methanothermobacter thermautotrophicus
7.5
-
acetate pH 7.0, 65°C, mutant enzyme Y498A Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus Q2I0L7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA important role for motif III (498YTAGD502) in catalysis. The highly conserved Tyr in the first position may play a key role in active-site architecture through interaction with a highly conserved active-site Gln. The invariant Asp in the fifth position plays a critical role in ATP binding and catalysis through interaction with the 2'- and 3'-OH groups of the ribose moiety of ATP Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
?

Synonyms

Synonyms Comment Organism
ACS1
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
ATP pH 7.0, 65°C, mutant enzyme G501A Methanothermobacter thermautotrophicus
0.3
-
CoA pH 7.0, 65°C, mutant enzyme G501A Methanothermobacter thermautotrophicus
0.3
-
acetate pH 7.0, 65°C, mutant enzyme G501A Methanothermobacter thermautotrophicus
0.8
-
ATP pH 7.0, 65°C, mutant enzyme T499A Methanothermobacter thermautotrophicus
0.8
-
acetate pH 7.0, 65°C, mutant enzyme T499A Methanothermobacter thermautotrophicus
0.8
-
CoA pH pH 7.0, 65°C, mutant enzyme T499A Methanothermobacter thermautotrophicus
1.5
-
ATP pH 7.0, 65°C, mutant enzyme A500T Methanothermobacter thermautotrophicus
1.5
-
CoA pH 7.0, 65°C, mutant enzyme A500T Methanothermobacter thermautotrophicus
1.5
-
acetate pH 7.0, 65°C, mutant enzyme A500T Methanothermobacter thermautotrophicus
1.6
-
ATP pH 7.0, 65°C, mutant enzyme Y498A Methanothermobacter thermautotrophicus
1.6
-
CoA pH 7.0, 65°C, mutant enzyme Y498A Methanothermobacter thermautotrophicus
1.6
-
acetate pH 7.0, 65°C, mutant enzyme Y498A Methanothermobacter thermautotrophicus
66.6
-
ATP pH 7.0, 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
66.6
-
CoA pH 7.0, 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
66.6
-
acetate pH 7.0, 65°C, wild-type enzyme Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 assay at Methanothermobacter thermautotrophicus