Activating Compound | Comment | Organism | Structure |
---|---|---|---|
reduced ferredoxin | required | Moorella thermoacetica |
Cloned (Comment) | Organism |
---|---|
expression of C-terminally His-tagged ACS in Escherichia coli strain BL21(DE3) | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Moorella thermoacetica | reaction of acetylated ACS with CoA and Fd-II, a step of the catalytic cycle in which the acetylated ACS reacts with CoA to form acetyl-CoA | AMP + diphosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged ACS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under anaerobic conditions | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Moorella thermoacetica | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | reaction of acetylated ACS with CoA and Fd-II, a step of the catalytic cycle in which the acetylated ACS reacts with CoA to form acetyl-CoA | Moorella thermoacetica | AMP + diphosphate + acetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Acetyl-CoA synthase | - |
Moorella thermoacetica |
ACS | - |
Moorella thermoacetica |
More | acetyl-CoA synthase is a subunit of the bifunctional CO dehydrogenase/acetyl-CoA synthase, CODH/ACS, complex | Moorella thermoacetica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
CO/acetyl-CoA exchange assay at | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
CO/acetyl-CoA exchange assay at | Moorella thermoacetica |
General Information | Comment | Organism |
---|---|---|
metabolism | acetyl-CoA synthase, a subunit of the bifunctional CO dehydrogenase/acetyl-CoA synthase, CODH/ACS, complex of Moorella thermoacetica requires reductive activation in order to catalyze acetyl-CoA synthesis and related partial reactions, including the CO/acetyl-CoA exchange reaction. Ferredoxin(II), which harbors two [4Fe-4S] clusters and is an electron acceptor for CODH, serves as a redox activator of ACS. Ferredoxin interfaces with an internal redox shuttle in acetyl-CoA synthase during reductive activation and catalysis. The midpoint reduction potential for the catalytic one-electron redoxactive species in the CO/acetyl-CoAexchange reaction is -511 mV. Incubation of ACS with Fd-II and CO leads to the formation of the NiFeC species. Mechanism, overview | Moorella thermoacetica |