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Literature summary for 6.2.1.1 extracted from

  • Brasen, C.; Urbanke, C.; Schonheit, P.
    A novel octameric AMP-forming acetyl-CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (2005), FEBS Lett., 579, 477-482.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
8 * 75000, SDS-PAGE Pyrobaculum aerophilum
610000
-
analytical ultracentrifugation Pyrobaculum aerophilum
625000
-
gel filtration Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
Pyrobaculum aerophilum AMP + diphosphate + acetyl-CoA
-
r
ATP + butyrate + CoA 25% of the activity with acetate Pyrobaculum aerophilum AMP + diphosphate + butyryl-CoA
-
?
ATP + formate + CoA 27% of the activity with acetate Pyrobaculum aerophilum AMP + diphosphate + formyl-CoA
-
?
ATP + isobutyrate + CoA 28% of the activity with acetate Pyrobaculum aerophilum AMP + diphosphate + isobutyryl-CoA
-
?
ATP + propanoate + CoA 118% of the activity with acetate Pyrobaculum aerophilum AMP + diphosphate + propanoyl-CoA
-
?

Subunits

Subunits Comment Organism
octamer 8 * 75000, SDS-PAGE Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
97
-
above Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
120 min, stable Pyrobaculum aerophilum
98
-
half-life: 72 min Pyrobaculum aerophilum
100
-
half-life: 8 min. Addition of 1 M (NH4)2SO4 stabilizes the enzyme to a half-life of 24 min Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Pyrobaculum aerophilum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 60% of maximal activity, pH 8.5: about 50% of maximal activity Pyrobaculum aerophilum