Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
8 * 75000, SDS-PAGE | Pyrobaculum aerophilum |
610000 | - |
analytical ultracentrifugation | Pyrobaculum aerophilum |
625000 | - |
gel filtration | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Pyrobaculum aerophilum | AMP + diphosphate + acetyl-CoA | - |
r | |
ATP + butyrate + CoA | 25% of the activity with acetate | Pyrobaculum aerophilum | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + formate + CoA | 27% of the activity with acetate | Pyrobaculum aerophilum | AMP + diphosphate + formyl-CoA | - |
? | |
ATP + isobutyrate + CoA | 28% of the activity with acetate | Pyrobaculum aerophilum | AMP + diphosphate + isobutyryl-CoA | - |
? | |
ATP + propanoate + CoA | 118% of the activity with acetate | Pyrobaculum aerophilum | AMP + diphosphate + propanoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 75000, SDS-PAGE | Pyrobaculum aerophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
97 | - |
above | Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
120 min, stable | Pyrobaculum aerophilum |
98 | - |
half-life: 72 min | Pyrobaculum aerophilum |
100 | - |
half-life: 8 min. Addition of 1 M (NH4)2SO4 stabilizes the enzyme to a half-life of 24 min | Pyrobaculum aerophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Pyrobaculum aerophilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH 5.5: about 60% of maximal activity, pH 8.5: about 50% of maximal activity | Pyrobaculum aerophilum |