Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | - |
Saccharomyces cerevisiae | |
Cl- | - |
Saccharomyces cerevisiae | |
monovalent cations | at 200 mM | Saccharomyces cerevisiae | |
SO42- | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
CoA | - |
Saccharomyces cerevisiae | |
0.28 | - |
acetate | - |
Saccharomyces cerevisiae | |
1.2 | - |
ATP | - |
Saccharomyces cerevisiae | |
10 | - |
propanoate | - |
Saccharomyces cerevisiae | |
17 | - |
Acrylate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae | |
Mg2+ | Km: 4.0 mM | Saccharomyces cerevisiae | |
Mn2+ | 10 mM, 38% of the activity relative to Mg2+. Progressive inactivation of the enzyme by MnCl2 is not reversible by subsequent addition of MgCl2 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Saccharomyces cerevisiae |
151000 | - |
sedimentation equilibrium analysis | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA | ordered bi uni uni bi ping-pong mechanism with ordered substrate addition and release | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 0.5 M potassium phosphate, pH 7.5, 7 mM 2-mercaptoethanol and 0.5 mM EDTA, protein concentration above 5 mg/ml, no loss of activity after 6 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acrylate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + acryloyl-CoA | - |
? | |
ATP + propanoate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + propanoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.8 | about 50% of maximal activity at pH 6.8 and 8.8 | Saccharomyces cerevisiae |
7.3 | 8.1 | 90% of maximal activity at pH 7.3 and 8.1 | Saccharomyces cerevisiae |