Literature summary for 6.1.1.9 extracted from

Fukai, S.; Nureki, O.; Sekine, S.; Shimada, A.; Tao, J.; Vassylyev, D.G.; Yokoyama, S.
Structural basis for double-sieve discrimination of L-valine from L-isoleucine and L-threonine by the complex of tRNA(Val) and valyl-tRNA synthetase (2000), Cell, 103, 793-803.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with tRNAValCAC isoacceptor and an analogue of the Val-adenylate intermediate, hanging-drop vapour diffusion method, 2 months, 4°C, 7-10 mg/ml protein/tRNA/Val-AMS solution in a molar ration of 1:1.1:2, plus equal volume of crystallization solution containing 50 mM sodium cacodylate, pH 6.5, 1.0 ammonium sulfate, 10 mM MgSO4, 6% 1,8-diaminooctane, equilibration against a reservoir solution of 50 mM sodium cacodylate, pH 6.5, 2.8 M ammonium sulfate, 10 mM MgSO4, X-ray structure determination at 2.9 A resolution and analysis	Thermus thermophilus

Crystallization (Comment)

Organism

enzyme in complex with tRNAValCAC isoacceptor and an analogue of the Val-adenylate intermediate, hanging-drop vapour diffusion method, 2 months, 4°C, 7-10 mg/ml protein/tRNA/Val-AMS solution in a molar ration of 1:1.1:2, plus equal volume of crystallization solution containing 50 mM sodium cacodylate, pH 6.5, 1.0 ammonium sulfate, 10 mM MgSO4, 6% 1,8-diaminooctane, equilibration against a reservoir solution of 50 mM sodium cacodylate, pH 6.5, 2.8 M ammonium sulfate, 10 mM MgSO4, X-ray structure determination at 2.9 A resolution and analysis

Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-valine + tRNAVal	Thermus thermophilus	-	AMP + diphosphate + L-valyl-tRNAVal	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-valine + tRNAVal = AMP + diphosphate + L-valyl-tRNAVal	pre- and post-transfer editing mechanism, mechanism, active site/editing site	Thermus thermophilus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-valine + tRNAVal	-	Thermus thermophilus	AMP + diphosphate + L-valyl-tRNAVal	-	?
ATP + L-valine + tRNAVal	the enzyme is strictly specific for the cognate L-valine and discriminates from the larger L-isoleucine and L-threonine by the tRNA-dependent double sieve mechanism	Thermus thermophilus	AMP + diphosphate + L-valyl-tRNAVal	-	?

Synonyms

Synonyms	Comment	Organism
G7a	-	Thermus thermophilus
Synthetase, valyl-transfer ribonucleate	-	Thermus thermophilus
Valine transfer ribonucleate ligase	-	Thermus thermophilus
Valine translase	-	Thermus thermophilus
Valine--tRNA ligase	-	Thermus thermophilus
ValRS	-	Thermus thermophilus
Valyl transfer ribonucleic acid synthetase	-	Thermus thermophilus
Valyl-transfer ribonucleate synthetase	-	Thermus thermophilus
Valyl-transfer RNA synthetase	-	Thermus thermophilus
Valyl-tRNA ligase	-	Thermus thermophilus
Valyl-tRNA synthetase	-	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Thermus thermophilus