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Literature summary for 6.1.1.9 extracted from

  • Sarantoglou, V.; Imbault, P.; Weil, J.H.
    The use of affinity elution from blue dextran Sepharose by yeast tRNA2Val in the complete purification of the cytoplasmic valyl-tRNA synthetase from Euglena gracilis (1980), Biochem. Biophys. Res. Commun., 93, 134-140.
    View publication on PubMed
Search for more literature for 6.1.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00005
-
 tRNAVal of Euglena gracilis Euglena gracilis
0.00008
-
 tRNAVal of Euglena, , cytoplasmic enzyme Euglena gracilis
0.00018
-
 tRNAVal substrate from cytoplasm, , cytoplasmic enzyme Euglena gracilis
0.05
-
 L-valine
-
 Euglena gracilis
0.07
-
 ATP
-
 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Euglena gracilis 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
126000
-
 gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.38
-
-
 Euglena gracilis

Storage Stability

Storage Stability Organism
4°C, 1 month, less than 20% loss of activity Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
-
 Euglena gracilis AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 126000, urea-SDS-PAGE Euglena gracilis