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Literature summary for 6.1.1.9 extracted from

  • Imbault, P.; Sarantoglou, V.; Weil, J.H.
    Properties of purified chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis (1982), Phytochemistry, 21, 1189-1193.
No PubMed abstract available
Search for more literature for 6.1.1.9

Activating Compound

Activating Compound Comment Organism Structure
spermine chloroplastic enzyme: unable to promote aminoacylation in absence of Mg2+. In presence of suboptimal concentrations of Mg2+, spermine can restore maximal activity (not in cytoplasmic enzyme) Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
KCl above 30 mM: inhibits activity of cytoplasmic enzyme more strongly than that of chloroplastic enzyme, 30 mM: enhances activity of the cytoplasmic enzyme, has no effect on the chloroplastic enzyme Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00005
-
 tRNAVal of Euglena gracilis Euglena gracilis
0.00005
-
 tRNAVal cytoplasmic enzyme Euglena gracilis
0.00006
-
 tRNAVal of Euglena gracilis, , chloroplastic enzyme Euglena gracilis
0.00008
-
 tRNAVal tRNA, of E. coli, , chloroplasmic enzyme Euglena gracilis
0.00008
-
 tRNAVal tRNA, of yeast, , cytoplasmic enzyme Euglena gracilis
0.015
-
 L-valine chloroplastic enzyme Euglena gracilis
0.05
-
 L-valine cytoplasmic enzyme Euglena gracilis
0.05
-
 L-valine ATP, cytoplasmic enzyme Euglena gracilis
0.07
-
 ATP cytoplasmic enzyme Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Euglena gracilis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no activity Euglena gracilis
Co2+ can partially replace Mg2+ in reaction with chloroplastic enzyme only Euglena gracilis
KCl 30 mM: enhances activity of the cytoplasmic enzyme, has no effect on the chloroplastic enzyme, above 30 mM: inhibits activity of cytoplasmic enzyme more strongly than that of chloroplastic enzyme Euglena gracilis
Mg2+ divalent cation required, Mg2+ is most effective Euglena gracilis
Mg2+ optimal Mg2+/ATP ratio is 2.5 for the chloroplastic and 1 for the cytoplasmic enzyme Euglena gracilis
Mn2+ 60% of the activation with Mg2+ Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal chloroplastic enzyme charges E. coli tRNA and not yeast tRNA, cytoplasmic enzyme is able to aminoacylate yeast tRNA but not E. coli tRNA Euglena gracilis AMP + diphosphate + L-valyl-tRNAVal
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 Tris-HCl or cacodylate buffer Euglena gracilis