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Literature summary for 6.1.1.7 extracted from
- Guanidine hydrochloride mediated denaturation of E. coli alanyl-tRNA Synthetase: identification of an inactive dimeric intermediate (2014), Protein J., 33, 119-127.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| guanidine hydrochloride | at 0.8 M guanidine hydrochloride, the enzyme forms a molten globule like intermediate, which is enzymatically inactive | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tRNAAla | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography, and gel filtration | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| a complete reversibility of enzyme activity is observed on refolding by dilution from 5 to 0.5 M guanidine hydrochloride | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alanyl-tRNA synthetase | - |
Escherichia coli |
| AlaRS | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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