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Literature summary for 6.1.1.7 extracted from

  • Dignam, J.D.; Guo, J.; Griffith, W.P.; Garbett, N.C.; Holloway, A.; Mueser, T.
    Allosteric interaction of nucleotides and tRNA(Ala) with E. coli alanyl-tRNA synthetase (2011), Biochemistry, 50, 9886-9900.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3star cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5'-O-[N-(L-alanyl)-sulfamoyl]adenosine
-
Escherichia coli
5'-O-[N-(L-glycinyl)sulfamoyl]-adenosine
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
245000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-alanine + tRNAAla Escherichia coli
-
AMP + diphosphate + L-alanyl-tRNAAla
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Superose 12 column chromatography, Q Sepharose column chromatography and Sephacryl S200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + tRNAAla
-
Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
?

Synonyms

Synonyms Comment Organism
Alanyl-tRNA synthetase
-
Escherichia coli
AlaRS
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000002
-
5'-O-[N-(L-alanyl)-sulfamoyl]adenosine in 20 mM Tris-HCl (pH 8.0), at 30°C Escherichia coli
0.000004
-
5'-O-[N-(L-glycinyl)sulfamoyl]-adenosine in 20 mM Tris-HCl (pH 8.0), at 30°C Escherichia coli