Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21DE3star cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[N-(L-alanyl)-sulfamoyl]adenosine | - |
Escherichia coli | |
5'-O-[N-(L-glycinyl)sulfamoyl]-adenosine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
245000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Superose 12 column chromatography, Q Sepharose column chromatography and Sephacryl S200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alanyl-tRNA synthetase | - |
Escherichia coli |
AlaRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000002 | - |
5'-O-[N-(L-alanyl)-sulfamoyl]adenosine | in 20 mM Tris-HCl (pH 8.0), at 30°C | Escherichia coli | |
0.000004 | - |
5'-O-[N-(L-glycinyl)sulfamoyl]-adenosine | in 20 mM Tris-HCl (pH 8.0), at 30°C | Escherichia coli |