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Literature summary for 6.1.1.7 extracted from

  • Ishijima, J.; Uchida, Y.; Kuroishi, C.; Tuzuki, C.; Takahashi, N.; Okazaki, N.; Yutani, K.; Miyano, M.
    Crystal structure of alanyl-tRNA synthetase editing-domain homolog (PH0574) from a hyperthermophile, Pyrococcus horikoshii OT3 at 1.45 A resolution (2006), Proteins, 62, 1133-1137.
    View publication on PubMed
Search for more literature for 6.1.1.7

Cloned(Commentary)

Cloned (Comment) Organism
gene PH0574, expression in Escherichia coli strain BL21 Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-labeld AlaRS editing-domain homolog, PH0574, 37.6 mg/ml protein, 27.5%w/v PEG 4000, and 100 mM MES-Na, pH 6.3, using a full-automatic protein crystallization and observation system, X-ray diffraction structure determination and analysis at 1.45 A resolution Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Pyrococcus horikoshii
Zn2+ active site bound by His9, His13, His120, and Cys116, involved in the catalytic reaction Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18152
-
 1 * 18152, crystal structure analysis, two-domain structure consisting of seven antiparallel beta-sheets and six helices Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-alanine + tRNAAla Pyrococcus horikoshii
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
ATP + L-alanine + tRNAAla Pyrococcus horikoshii OT-3
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58307 strain OT3, gene PH0574
-
Pyrococcus horikoshii OT-3 O58307 strain OT3, gene PH0574
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PH0574, from Escherichia coli strain BL21 by two steps of anion exchange chromatography, hydroxyapatite chromatography, and gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + tRNAAla
-
 Pyrococcus horikoshii AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
ATP + L-alanine + tRNAAla AlaRS hydrolyzes mischarged tRNA by the catalytic zinc ion in the editing domain, modeling, overview Pyrococcus horikoshii AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
ATP + L-alanine + tRNAAla
-
 Pyrococcus horikoshii OT-3 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
ATP + L-alanine + tRNAAla AlaRS hydrolyzes mischarged tRNA by the catalytic zinc ion in the editing domain, modeling, overview Pyrococcus horikoshii OT-3 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 18152, crystal structure analysis, two-domain structure consisting of seven antiparallel beta-sheets and six helices Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
Alanyl-tRNA synthetase
-
 Pyrococcus horikoshii
AlaRS
-
 Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pyrococcus horikoshii