Literature summary for 6.1.1.7 extracted from

Huang, H.Y.; Tang, H.L.; Chao, H.Y.; Yeh, L.S.; Wang, C.C.
An unusual pattern of protein expression and localization of yeast alanyl-tRNA synthetase isoforms (2006), Mol. Microbiol., 60, 189-198.

Cloned(Commentary)

Cloned (Comment)	Organism
gene ALA1, DNA and amino acid sequence determination and analysis, mitochondrial and cytoplasmic isozymes are encoded by a single nuclear gene ALA1, through alternative use of inframe successive ACG triplets and a downstream AUG triplet, overview, despite participation of the non-AUG-initiated leader peptide in mitochondrial localization, the leader peptide per se cannot target a cytoplasmic passenger protein into mitochondria under normal conditions, functional mapping	Saccharomyces cerevisiae

Cloned (Comment)

Organism

gene ALA1, DNA and amino acid sequence determination and analysis, mitochondrial and cytoplasmic isozymes are encoded by a single nuclear gene ALA1, through alternative use of inframe successive ACG triplets and a downstream AUG triplet, overview, despite participation of the non-AUG-initiated leader peptide in mitochondrial localization, the leader peptide per se cannot target a cytoplasmic passenger protein into mitochondria under normal conditions, functional mapping

Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of the cytoplasmic isozyme enables it to overcome the compartmental barrier and function in the mitochondria as well, but deletion of as few as eight amino acid residues from its amino-terminus eliminates such a potential, the sequence upstream of the first in-frame AUG initiator not only carries an unusual initiation site, but also contributes to the pattern of protein expression and localization	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Saccharomyces cerevisiae	5737	-
mitochondrion	very poor mitochondrial targeting activity of the ALA1 leader peptide, mapping of the mitochondrial targeting signal, overview	Saccharomyces cerevisiae	5739	-
additional information	isozyme pattern of expression and localization, overview	Saccharomyces cerevisiae	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-alanine + tRNAAla	Saccharomyces cerevisiae	-	AMP + diphosphate + L-alanyl-tRNAAla	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	mitochondrial and cytoplasmic isozymes encoded by a single nuclear gene ALA1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	isozyme pattern of expression and localization, overview	Saccharomyces cerevisiae	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-alanine + tRNAAla	-	Saccharomyces cerevisiae	AMP + diphosphate + L-alanyl-tRNAAla	-	?

Synonyms

Synonyms	Comment	Organism
Alanyl-tRNA synthetase	-	Saccharomyces cerevisiae
AlaRS	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae