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Literature summary for 6.1.1.7 extracted from
- Mapping contacts between Escherichia coli alanyl tRNA synthetase and 2' hydroxyls using a complete tRNA molecule (2000), Biochemistry, 39, 8250-8258.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
tRNAAla | wild-type and mutant substrate from Escherichia coli, pH 7.5, 37°C | Escherichia coli |  |
| 0.0019 | - |
tRNAPhe(17) | tRNAPhe mutant tRNAPhe(17), pH 7.5, 37°C | Escherichia coli | |
| 0.0021 | - |
tRNAPhe | native dual specific tRNAPhe derivative substrate from Saccharomyces cerevisiae, pH 7.5, 37°C | Escherichia coli | |
| 0.0031 | - |
tRNAPhe(38) | tRNAPhe mutant tRNAPhe(38), pH 7.5, 37°C | Escherichia coli | |
| 0.017 | - |
tRNAPhe(57) | tRNAPhe mutant tRNAPhe(57), pH 7.5, 37°C | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tRNAAla | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
purified recombinant His-tagged enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + dual specific tRNAPhe | effects of deoxynucleotide substitutions in the tRNAPhe substrate on the enzyme activity, overview | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPhe | - |
? | |
| ATP + L-alanine + dual specific tRNAPhe(17) | site-specific modification leads to deoxynucleotide substituted yeast tRNA substrate, the backbone is interrupted on the 3'-side of nucleotide 17 | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPhe(17) | - |
? | |
| ATP + L-alanine + dual specific tRNAPhe(38) | site-specific modification leads to deoxynucleotide substituted yeast tRNA substrate, the backbone is interrupted on the 3'-side of nucleotide 38 | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPhe(38) | - |
? | |
| ATP + L-alanine + dual specific tRNAPhe(57) | site-specific modification leads to deoxynucleotide substituted yeast tRNA substrate, the backbone is interrupted on the 3'-side of nucleotide 57 | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPhe(57) | - |
? | |
| ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
| ATP + L-alanine + tRNAAla | wild-type and mutant Escherichia coli tRNAAla substrates, enzyme interacts with 2'-hydroxyls in the acceptor stem of the tRNA substrate | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
| ATP + L-alanine + tRNAPhe(17) | - |
Escherichia coli | ? | - |
? | |
| ATP + L-alanine + tRNAPhe(38) | - |
Escherichia coli | ? | - |
? | |
| ATP + L-alanine + tRNAPhe(57) | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ala-tRNA synthetase | - |
Escherichia coli |
| Alanine transfer RNA synthetase | - |
Escherichia coli |
| Alanine translase | - |
Escherichia coli |
| Alanine tRNA synthetase | - |
Escherichia coli |
| Alanine-transfer RNA ligase | - |
Escherichia coli |
| alanine-tRNA ligase | - |
Escherichia coli |
| Alanyl-transfer ribonucleate synthetase | - |
Escherichia coli |
| Alanyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
| Alanyl-transfer RNA synthetase | - |
Escherichia coli |
| Alanyl-tRNA synthetase | - |
Escherichia coli |
| AlaRS | - |
Escherichia coli |
| Synthase, alanyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
tRNAPhe(57) | tRNAPhe mutant tRNAPhe(57), pH 7.5, 37°C | Escherichia coli | |
| 0.19 | - |
tRNAAla | mutant substrate from Escherichia coli, pH 7.5, 37°C | Escherichia coli | |
| 0.25 | - |
tRNAPhe | native dual specific tRNAPhe substrate from Saccharomyces cerevisiae, pH 7.5, 37°C | Escherichia coli | |
| 0.25 | - |
tRNAPhe(17) | tRNAPhe mutant tRNAPhe(17), pH 7.5, 37°C | Escherichia coli | |
| 0.44 | - |
tRNAAla | wild-type substrate from Escherichia coli, pH 7.5, 37°C | Escherichia coli | |
| 0.46 | - |
tRNAPhe(38) | tRNAPhe mutant tRNAPhe(38), pH 7.5, 37°C | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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