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Literature summary for 6.1.1.7 extracted from
- Glycyl- and alanyl-tRNA synthetases from Bombyx mori. Purification and properties (1984), J. Biol. Chem., 259, 4043-4048.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermine | stimulates only at low Mg2+ concentrations | Bombyx mori |  |
General Stability
| General Stability | Organism |
|---|---|
| sensitive to repeated freezing and thawing | Bombyx mori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00063 | - |
liver tRNA | Bombyx mori tRNA | Bombyx mori | |
| 0.34 | - |
alanine | - |
Bombyx mori | |
| 1.7 | - |
ATP | - |
Bombyx mori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimum: 5-10 mM | Bombyx mori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
1 * 110000, SDS-PAGE | Bombyx mori |
| 151000 | - |
gel filtration | Bombyx mori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alanine + tRNAAla | Bombyx mori | key role in protein biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| silk gland | posterior | Bombyx mori | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 3 months, retains approximately 90% of activity | Bombyx mori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alanine + tRNAAla | key role in protein biosynthesis | Bombyx mori | ? | - |
? | |
| ATP + L-alanine + liver tRNA | - |
Bombyx mori | ? | - |
? | |
| ATP + L-alanine + tRNAAla | - |
Bombyx mori | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 110000, SDS-PAGE | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
alanyl-tRNA synthesis | Bombyx mori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 7.5 | 6.4: about 60% of maximal activity, 7.5: optimum | Bombyx mori |
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