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Literature summary for 6.1.1.6 extracted from

  • Samuelsson, T.; Lundvik, L.
    Purification and some properties of asparagine, lysine, serine, and valine:tRNA ligases from Bacillus stearothermophilus (1978), J. Biol. Chem., 253, 7033-7039.
    View publication on PubMed
Search for more literature for 6.1.1.6

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 2 * 58000, SDS-PAGE Geobacillus stearothermophilus
109000
-
 gel filtration Geobacillus stearothermophilus
112000
-
 equilibrium sedimentation measurement Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.345
-
-
 Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
-80°C, 0.1 M potassium phosphate buffer, pH 7.0 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + lysine + tRNALys
-
 Geobacillus stearothermophilus AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 after 4 h complete loss of aminoacylation activity, 3 min, stable in presence of all substrates except lysine Geobacillus stearothermophilus
70
-
 0.5 min, stable in presence of all substrates except lysine Geobacillus stearothermophilus