Crystallization (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
109000 | - |
gel filtration | Geobacillus stearothermophilus |
112000 | - |
equilibrium sedimentation measurement | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.345 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-80°C, 0.1 M potassium phosphate buffer, pH 7.0 | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
after 4 h complete loss of aminoacylation activity, 3 min, stable in presence of all substrates except lysine | Geobacillus stearothermophilus |
70 | - |
0.5 min, stable in presence of all substrates except lysine | Geobacillus stearothermophilus |