Protein Variants | Comment | Organism |
---|---|---|
E246D | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
E246R | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
E264A | the mutant shows more than 90% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
E264K | the mutant catalyses the production of glycerol-3-phosphate, powered by ATP turnover to ADP but shows little formation of diadenosine tri- and tetraphosphates under normal conditions (additional Zn2+/L-lysine/Mg2+) | Escherichia coli |
E264N | the mutant catalyses the production of glycerol-3-phosphate, powered by ATP turnover to ADP but shows little formation of diadenosine tri- and tetraphosphates under normal conditions (additional Zn2+/L-lysine/Mg2+) | Escherichia coli |
E264Q | the mutant catalyses the production of glycerol-3-phosphate, powered by ATP turnover to ADP but shows little formation of diadenosine tri- and tetraphosphates under normal conditions (additional Zn2+/L-lysine/Mg2+) | Escherichia coli |
E273A | the mutant shows more than 90% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
E414A | the mutant shows more than 90% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
E421A | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
F261A | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
F274A | the mutant shows more than 90% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
G265A | the mutant shows more than 90% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
H270A | the mutant catalyses the production of glycerol-3-phosphate, powered by ATP turnover to ADP but shows little formation of diadenosine tri- and tetraphosphates under normal conditions (additional Zn2+/L-lysine/Mg2+) | Escherichia coli |
I266A | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
N260A | inactive | Escherichia coli |
N263A | inactive | Escherichia coli |
N271A | inactive | Escherichia coli |
P272A | the mutant shows more than 90% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
R262A | inactive | Escherichia coli |
R269A | inactive | Escherichia coli |
R269E | inactive | Escherichia coli |
R269K | inactive | Escherichia coli |
R269Q | inactive | Escherichia coli |
R480A | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
S267A | the mutant shows more than 50% loss in catalytic efficiency compared to the wild type enzyme | Escherichia coli |
V268A | inactive | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, the wild-type enzyme has a weaker glycerol kinase-like capability in the absence of Zn2+ and is dominated by diadenosine tri- and tetraphosphate synthesis in its presence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Escherichia coli | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, and Sephacryl S300 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Escherichia coli | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
additional information | the enzyme also converts ATP to diadenosine tri- and tetraphosphates in the presence of L-lysine/Mg2+/Zn2+ | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LysU | - |
Escherichia coli |
Lysyl-tRNA synthetase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |