Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 2% (v/v) tascimate pH 6.0, 20% (w/v) PEG 3350 | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Plasmodium falciparum | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
2 * 65000, SDS-PAGE | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Plasmodium falciparum | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IDJ8 | - |
- |
Purification (Comment) | Organism |
---|---|
amylose bead chromatography and S200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Plasmodium falciparum | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
additional information | the enzyme is also capable of synthesizing the signalling molecule diadenosine tetraphosphate using ATP as a substrate in the presence of L-Lys and Zn2+ | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 65000, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
KRS-1 | - |
Plasmodium falciparum |
LysRS | - |
Plasmodium falciparum |
Lysyl-tRNA synthetase | - |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |