Cloned (Comment) | Organism |
---|---|
expressed in the bacterial strain BL21 (DE3) CodonPlus using the vectors pET20b, both DELTAS70-T584 and full length LysRS | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
micrositting drop-vapor diffusion method, both DELTAS70-T584 and full length LysRS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTAS70-T584 | truncation at S70-T584 and full length LysRS (M1-V597) expressed, purified, and attempted for crystallization | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
LysRSalpha2, Superdex-200 gel-filtration, results show clearly a dynamic dimer-tetramer oligomerization for LysRS | Homo sapiens |
240000 | - |
LysRSalpha4, Superdex-200 gel-filtration, results show clearly a dynamic dimer-tetramer oligomerization for LysRS | Homo sapiens |
270000 | - |
calculated mass, alpha2:alpha2 tetramer. This tetramer is probably functional for aminoacylation, because docking of tRNA molecules to the tetrameric LysRS seems not to cause steric clash | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q15046 | sequence alignment of class II LysRS from Homo sapiens, Drosophila melanogaster, Saccharomyces cerevisiae, and Escherichia coli shown | - |
Purification (Comment) | Organism |
---|---|
purified to homogeneity by Ni-NTA affinity column and a Q highperformance column, both DELTAS70-T584 and full length LysRS | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Homo sapiens | AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | it is not understood, how the two dimeric proteins combine to make a presumptive alpha2beta2 heterotetramer | Homo sapiens |
homodimer | - |
Homo sapiens |
homotetramer | alpha2:alpha2 tetramer. This tetramer is probably functional for aminoacylation, because docking of tRNA molecules to the tetrameric LysRS seems not to cause steric clash | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LysRS | class II | Homo sapiens |
Lysyl-tRNA synthetase | is bound to the multisynthetase complex | Homo sapiens |