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Literature summary for 6.1.1.6 extracted from

  • Guo, M.; Ignatov, M.; Musier-Forsyth, K.; Schimmel, P.; Yang, X.L.
    Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation (2008), Proc. Natl. Acad. Sci. USA, 105, 2331-2336.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the bacterial strain BL21 (DE3) CodonPlus using the vectors pET20b, both DELTAS70-T584 and full length LysRS Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
micrositting drop-vapor diffusion method, both DELTAS70-T584 and full length LysRS Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTAS70-T584 truncation at S70-T584 and full length LysRS (M1-V597) expressed, purified, and attempted for crystallization Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
LysRSalpha2, Superdex-200 gel-filtration, results show clearly a dynamic dimer-tetramer oligomerization for LysRS Homo sapiens
240000
-
LysRSalpha4, Superdex-200 gel-filtration, results show clearly a dynamic dimer-tetramer oligomerization for LysRS Homo sapiens
270000
-
calculated mass, alpha2:alpha2 tetramer. This tetramer is probably functional for aminoacylation, because docking of tRNA molecules to the tetrameric LysRS seems not to cause steric clash Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15046 sequence alignment of class II LysRS from Homo sapiens, Drosophila melanogaster, Saccharomyces cerevisiae, and Escherichia coli shown
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity by Ni-NTA affinity column and a Q highperformance column, both DELTAS70-T584 and full length LysRS Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
Homo sapiens AMP + diphosphate + L-lysyl-tRNALys
-
?

Subunits

Subunits Comment Organism
heterotetramer it is not understood, how the two dimeric proteins combine to make a presumptive alpha2beta2 heterotetramer Homo sapiens
homodimer
-
Homo sapiens
homotetramer alpha2:alpha2 tetramer. This tetramer is probably functional for aminoacylation, because docking of tRNA molecules to the tetrameric LysRS seems not to cause steric clash Homo sapiens

Synonyms

Synonyms Comment Organism
LysRS class II Homo sapiens
Lysyl-tRNA synthetase is bound to the multisynthetase complex Homo sapiens