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Literature summary for 6.1.1.6 extracted from
- Substrate-induced conformational changes of the truncated catalytic domain of Geobacillus stearothermophilus lysyl-tRNA synthetase as examined by fluorescence (2008), Biochim. Biophys. Acta, 1784, 1633-1640.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| CAT W314F | mutant of the truncated C-terminal catalytic domain CAT | Geobacillus stearothermophilus |
| CAT W332F | mutant of the truncated C-terminal catalytic domain CAT | Geobacillus stearothermophilus |
| GsLysRS W314F | mutant of Geobacillus stearothermophilus lysyl-tRNA synthetase | Geobacillus stearothermophilus |
| GsLysRS W332F | mutant of Geobacillus stearothermophilus lysyl-tRNA synthetase | Geobacillus stearothermophilus |
| truncated N-terminal tRNA anticodon-binding domain | TAB | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
wild-type CAT and CAT mutants, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-lysine + tRNALys | Geobacillus stearothermophilus | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q9RHV9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CAT mutants are purified to homogeneity | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | CAT mutants and wild-type CAT | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GsLysRS | - |
Geobacillus stearothermophilus |
| Lysyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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