Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3+ RPC) cells | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
Y491E | exhibits significant improvement compared to the wild type in aminoacylation of a tRNALysUUG | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
tRNALysUUU | wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP | Pyrococcus horikoshii | |
1.4 | - |
tRNALysCUU | wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP | Pyrococcus horikoshii | |
1.5 | - |
tRNALysGUU | wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MonoQ 5/5 column chromatography and Superose 12 gel filtration | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALysCUU | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysCUU + diphosphate | - |
? | |
ATP + L-lysine + tRNALysGUU | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysGUU + diphosphate | - |
? | |
ATP + L-lysine + tRNALysUCU | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysUCU + diphosphate | - |
? | |
ATP + L-lysine + tRNALysUGU | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysUGU + diphosphate | - |
? | |
ATP + L-lysine + tRNALysUUC | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysUUC + diphosphate | - |
? | |
ATP + L-lysine + tRNALysUUG | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysUUG + diphosphate | - |
? | |
ATP + L-lysine + tRNALysUUU | - |
Pyrococcus horikoshii | AMP + L-lysyl-tRNALysUUU + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class I lysyl-tRNA synthetase | - |
Pyrococcus horikoshii |
LysRS1 | - |
Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
tRNALysGUU | wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP | Pyrococcus horikoshii | |
0.062 | - |
tRNALysCUU | wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP | Pyrococcus horikoshii | |
0.44 | - |
tRNALysUUU | wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |