Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Borreliella burgdorferi |
Protein Variants | Comment | Organism |
---|---|---|
E43I | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
E43Q | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
G29A | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
H242A | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
H242L | shows almost no change in the catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
H242W | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
T31G | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
T31S | shows almost no change in the catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
W220A | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
W220L | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
W220Y | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
Y269F | 6fold drop in the catalytic efficiency compared to the wild type enzyme | Borreliella burgdorferi |
Y269S | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-(2-aminoethyl)-L-cysteine | competitive inhibitor | Borreliella burgdorferi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-lysine | mutant enzyme H242L | Borreliella burgdorferi | |
0.23 | - |
L-lysine | wild type enzyme | Borreliella burgdorferi | |
0.33 | - |
L-lysine | mutant enzyme W220A | Borreliella burgdorferi | |
0.4 | - |
L-lysine | mutant enzyme Y269F | Borreliella burgdorferi | |
0.76 | - |
L-lysine | mutant enzyme T31S | Borreliella burgdorferi | |
0.83 | - |
tRNALysUUU | mutant enzyme Y269F | Borreliella burgdorferi | |
1 | - |
tRNALysUUU | mutant enzyme H242A | Borreliella burgdorferi | |
1.1 | - |
tRNALysUUU | mutant enzyme W220L | Borreliella burgdorferi | |
1.1 | - |
tRNALysUUU | mutant enzyme Y269S | Borreliella burgdorferi | |
1.2 | - |
tRNALysUUU | mutant enzyme W220Y | Borreliella burgdorferi | |
1.3 | - |
ATP | wild type enzyme | Borreliella burgdorferi | |
1.3 | - |
tRNALysUUU | mutant enzyme H242L | Borreliella burgdorferi | |
1.6 | - |
ATP | mutant enzyme Y269F | Borreliella burgdorferi | |
1.7 | - |
ATP | mutant enzyme T31S | Borreliella burgdorferi | |
2 | - |
tRNALysUUU | wild type enzyme | Borreliella burgdorferi | |
2.1 | - |
tRNALysUUU | mutant enzyme T31G | Borreliella burgdorferi | |
2.1 | - |
tRNALysUUU | mutant enzyme T31S | Borreliella burgdorferi | |
2.5 | - |
tRNALysUUU | mutant enzyme W220A | Borreliella burgdorferi | |
2.8 | - |
L-lysine | mutant enzyme Y269S | Borreliella burgdorferi | |
3.3 | - |
L-lysine | mutant enzyme H242A | Borreliella burgdorferi | |
3.3 | - |
L-lysine | mutant enzyme W220L | Borreliella burgdorferi | |
4.4 | - |
L-lysine | mutant enzyme T31G | Borreliella burgdorferi | |
4.7 | - |
L-lysine | mutant enzyme W220Y | Borreliella burgdorferi | |
5.1 | - |
tRNALysUUU | mutant enzyme G29A | Borreliella burgdorferi | |
6.3 | - |
L-lysine | mutant enzyme G29A | Borreliella burgdorferi | |
6.9 | - |
ATP | mutant enzyme Y269S | Borreliella burgdorferi | |
9.5 | - |
ATP | mutant enzyme T31G | Borreliella burgdorferi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Borreliella burgdorferi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography Superose 6 column chromatography | Borreliella burgdorferi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALysUUU | - |
Borreliella burgdorferi | AMP + L-lysyl-tRNALysUUU + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class I lysyl-tRNA synthetase | - |
Borreliella burgdorferi |
LysRS1 | - |
Borreliella burgdorferi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
L-lysine | mutant enzyme H242A | Borreliella burgdorferi | |
0.00012 | - |
tRNALysUUU | mutant enzyme W220L | Borreliella burgdorferi | |
0.00013 | - |
tRNALysUUU | mutant enzyme H242A | Borreliella burgdorferi | |
0.00017 | - |
L-lysine | mutant enzyme W220L | Borreliella burgdorferi | |
0.00051 | - |
ATP | mutant enzyme Y269S | Borreliella burgdorferi | |
0.00056 | - |
tRNALysUUU | mutant enzyme W220A | Borreliella burgdorferi | |
0.00075 | - |
L-lysine | mutant enzyme W220A | Borreliella burgdorferi | |
0.0011 | - |
ATP | mutant enzyme T31G | Borreliella burgdorferi | |
0.0023 | - |
tRNALysUUU | mutant enzyme Y269S | Borreliella burgdorferi | |
0.0026 | - |
L-lysine | mutant enzyme Y269S | Borreliella burgdorferi | |
0.0046 | - |
L-lysine | mutant enzyme T31G | Borreliella burgdorferi | |
0.005 | - |
tRNALysUUU | mutant enzyme T31G | Borreliella burgdorferi | |
0.017 | - |
ATP | mutant enzyme Y269F | Borreliella burgdorferi | |
0.099 | - |
L-lysine | mutant enzyme Y269F | Borreliella burgdorferi | |
0.13 | - |
L-lysine | mutant enzyme W220Y | Borreliella burgdorferi | |
0.14 | - |
tRNALysUUU | mutant enzyme Y269F | Borreliella burgdorferi | |
0.23 | - |
tRNALysUUU | mutant enzyme W220Y | Borreliella burgdorferi | |
0.29 | - |
L-lysine | mutant enzyme H242L | Borreliella burgdorferi | |
0.29 | - |
tRNALysUUU | mutant enzyme H242L | Borreliella burgdorferi | |
0.33 | - |
tRNALysUUU | wild type enzyme | Borreliella burgdorferi | |
0.34 | - |
L-lysine | wild type enzyme | Borreliella burgdorferi | |
0.45 | - |
L-lysine | mutant enzyme G29A | Borreliella burgdorferi | |
0.53 | - |
L-lysine | mutant enzyme T31S | Borreliella burgdorferi | |
0.57 | - |
ATP | wild type enzyme | Borreliella burgdorferi | |
0.59 | - |
tRNALysUUU | mutant enzyme G29A | Borreliella burgdorferi | |
0.68 | - |
tRNALysUUU | mutant enzyme T31S | Borreliella burgdorferi | |
1.7 | - |
ATP | mutant enzyme T31S | Borreliella burgdorferi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Borreliella burgdorferi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme H242L | Borreliella burgdorferi | |
3.6 | - |
S-(2-aminoethyl)-L-cysteine | wild type enzyme | Borreliella burgdorferi | |
8.5 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme H242A | Borreliella burgdorferi | |
11 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme W220L | Borreliella burgdorferi | |
11.8 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme G29A | Borreliella burgdorferi | |
17 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme W220Y | Borreliella burgdorferi | |
19 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme W220A | Borreliella burgdorferi |